Effect of collagen digestion on the passive elastic properties of diaphragm muscle in rat

Justin Rowe; Qingshan Chen; Zachary J. Domire; Matthew B. McCullough; Gary Sieck; Wen Zhi Zhan; Kai Nan An

doi:10.1016/j.medengphy.2009.11.002

Effect of collagen digestion on the passive elastic properties of diaphragm muscle in rat

Justin Rowe, Qingshan Chen, Zachary J. Domire, Matthew B. McCullough, Gary Sieck, Wen Zhi Zhan, Kai Nan An

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Effects of collagen digestion have been defined up to the fibril level. However, the question remains as to whether the alteration of skeletal muscle extracellular matrix (ECM) affects a muscle's passive elastic response. Various elastography methods have been applied as tools for evaluating the mechanical properties and ECM content of skeletal muscle. In an effort to develop an ECM altered skeletal muscle model, this study determined the effect of collagen digestion on the passive elastic properties of skeletal muscle. Passive mechanical properties of rat diaphragms were evaluated in various degrees of collagen digestion. Between cyclic loading tests, muscle strips were immersed in various concentrations of clostridium histolyticum derived bacterial collagenase. All samples were later viewed via light microscopy. Cyclic testing revealed linear relationships between passive muscle stiffness and digestion time at multiple concentrations. These results demonstrate that collagenase digestion of the ECM in skeletal muscle could be used as a simple and reliable model of mechanically altered in vitro tissue samples.

Original language	English (US)
Pages (from-to)	90-94
Number of pages	5
Journal	Medical Engineering and Physics
Volume	32
Issue number	1
DOIs	https://doi.org/10.1016/j.medengphy.2009.11.002
State	Published - Jan 2010

Keywords

Biomechanics
Elastography
Extracellular matrix
Myopathy

ASJC Scopus subject areas

Biophysics
Biomedical Engineering

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10.1016/j.medengphy.2009.11.002

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title = "Effect of collagen digestion on the passive elastic properties of diaphragm muscle in rat",

abstract = "Effects of collagen digestion have been defined up to the fibril level. However, the question remains as to whether the alteration of skeletal muscle extracellular matrix (ECM) affects a muscle's passive elastic response. Various elastography methods have been applied as tools for evaluating the mechanical properties and ECM content of skeletal muscle. In an effort to develop an ECM altered skeletal muscle model, this study determined the effect of collagen digestion on the passive elastic properties of skeletal muscle. Passive mechanical properties of rat diaphragms were evaluated in various degrees of collagen digestion. Between cyclic loading tests, muscle strips were immersed in various concentrations of clostridium histolyticum derived bacterial collagenase. All samples were later viewed via light microscopy. Cyclic testing revealed linear relationships between passive muscle stiffness and digestion time at multiple concentrations. These results demonstrate that collagenase digestion of the ECM in skeletal muscle could be used as a simple and reliable model of mechanically altered in vitro tissue samples.",

keywords = "Biomechanics, Elastography, Extracellular matrix, Myopathy",

author = "Justin Rowe and Qingshan Chen and Domire, {Zachary J.} and McCullough, {Matthew B.} and Gary Sieck and Zhan, {Wen Zhi} and An, {Kai Nan}",

note = "Funding Information: This study is supported by National Institute of Health (NIH) grant EB00812 . The study sponsors had no role in study design, collection, analysis and interpretation of data, wiring of the manuscript, or decision to submit the manuscript for publication.",

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doi = "10.1016/j.medengphy.2009.11.002",

language = "English (US)",

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AU - Chen, Qingshan

AU - Domire, Zachary J.

AU - McCullough, Matthew B.

AU - Sieck, Gary

AU - Zhan, Wen Zhi

AU - An, Kai Nan

N1 - Funding Information: This study is supported by National Institute of Health (NIH) grant EB00812 . The study sponsors had no role in study design, collection, analysis and interpretation of data, wiring of the manuscript, or decision to submit the manuscript for publication.

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N2 - Effects of collagen digestion have been defined up to the fibril level. However, the question remains as to whether the alteration of skeletal muscle extracellular matrix (ECM) affects a muscle's passive elastic response. Various elastography methods have been applied as tools for evaluating the mechanical properties and ECM content of skeletal muscle. In an effort to develop an ECM altered skeletal muscle model, this study determined the effect of collagen digestion on the passive elastic properties of skeletal muscle. Passive mechanical properties of rat diaphragms were evaluated in various degrees of collagen digestion. Between cyclic loading tests, muscle strips were immersed in various concentrations of clostridium histolyticum derived bacterial collagenase. All samples were later viewed via light microscopy. Cyclic testing revealed linear relationships between passive muscle stiffness and digestion time at multiple concentrations. These results demonstrate that collagenase digestion of the ECM in skeletal muscle could be used as a simple and reliable model of mechanically altered in vitro tissue samples.

AB - Effects of collagen digestion have been defined up to the fibril level. However, the question remains as to whether the alteration of skeletal muscle extracellular matrix (ECM) affects a muscle's passive elastic response. Various elastography methods have been applied as tools for evaluating the mechanical properties and ECM content of skeletal muscle. In an effort to develop an ECM altered skeletal muscle model, this study determined the effect of collagen digestion on the passive elastic properties of skeletal muscle. Passive mechanical properties of rat diaphragms were evaluated in various degrees of collagen digestion. Between cyclic loading tests, muscle strips were immersed in various concentrations of clostridium histolyticum derived bacterial collagenase. All samples were later viewed via light microscopy. Cyclic testing revealed linear relationships between passive muscle stiffness and digestion time at multiple concentrations. These results demonstrate that collagenase digestion of the ECM in skeletal muscle could be used as a simple and reliable model of mechanically altered in vitro tissue samples.

KW - Biomechanics

KW - Elastography

KW - Extracellular matrix

KW - Myopathy

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Effect of collagen digestion on the passive elastic properties of diaphragm muscle in rat

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Keywords

ASJC Scopus subject areas

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