Effect of claudins 6 and 9 on paracellular permeability in MDCK II cells

David Sas, Mingchang Hu, Orson W. Moe, Michel Baum

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The neonatal proximal tubule has a lower permeability to chloride, higher resistance, and higher relative sodium-to-chloride permeability (P Na/PCl) than the adult tubule, which may be due to maturational changes in the tight junction. Claudins are tight-junction proteins between epithelial cells that determine paracellular permeability characteristics of epithelia. We have previously described the presence of two claudin isoforms, claudins 6 and 9, in the neonatal proximal tubule and subsequent reduction of these claudins during postnatal maturation. The question is whether changes in claudin expression are related to changes in functional characteristics in the neonatal tubule. We transfected claudins 6 and 9 into Madin-Darby canine kidney II (MDCK II) cells and performed electrophysiological studies to determine the resultant changes in physiological characteristics of the cells. Expression of claudins 6 and 9 resulted in an increased transepithelial resistance, decreased chloride permeability, and decreased PNa/PCl and PHCO3/PCl. These findings constitute the first characterization of the permeability characteristics of claudins 6 and 9 in a cell model and may explain why the neonatal proximal tubule has lower permeability to chloride and higher resistance than the adult proximal tubule.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume295
Issue number5
DOIs
StatePublished - Nov 1 2008
Externally publishedYes

Fingerprint

Claudins
Madin Darby Canine Kidney Cells
Permeability
Chlorides
Tight Junction Proteins
Tight Junctions
Sodium Chloride
Protein Isoforms
Epithelium
Epithelial Cells

Keywords

  • Neonate
  • Proximal tubule
  • Resistance

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Effect of claudins 6 and 9 on paracellular permeability in MDCK II cells. / Sas, David; Hu, Mingchang; Moe, Orson W.; Baum, Michel.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 295, No. 5, 01.11.2008.

Research output: Contribution to journalArticle

@article{1831956a4cdf4fce8db0c6df6554c20a,
title = "Effect of claudins 6 and 9 on paracellular permeability in MDCK II cells",
abstract = "The neonatal proximal tubule has a lower permeability to chloride, higher resistance, and higher relative sodium-to-chloride permeability (P Na/PCl) than the adult tubule, which may be due to maturational changes in the tight junction. Claudins are tight-junction proteins between epithelial cells that determine paracellular permeability characteristics of epithelia. We have previously described the presence of two claudin isoforms, claudins 6 and 9, in the neonatal proximal tubule and subsequent reduction of these claudins during postnatal maturation. The question is whether changes in claudin expression are related to changes in functional characteristics in the neonatal tubule. We transfected claudins 6 and 9 into Madin-Darby canine kidney II (MDCK II) cells and performed electrophysiological studies to determine the resultant changes in physiological characteristics of the cells. Expression of claudins 6 and 9 resulted in an increased transepithelial resistance, decreased chloride permeability, and decreased PNa/PCl and PHCO3/PCl. These findings constitute the first characterization of the permeability characteristics of claudins 6 and 9 in a cell model and may explain why the neonatal proximal tubule has lower permeability to chloride and higher resistance than the adult proximal tubule.",
keywords = "Neonate, Proximal tubule, Resistance",
author = "David Sas and Mingchang Hu and Moe, {Orson W.} and Michel Baum",
year = "2008",
month = "11",
day = "1",
doi = "10.1152/ajpregu.90596.2008",
language = "English (US)",
volume = "295",
journal = "American Journal of Physiology",
issn = "0363-6119",
publisher = "American Physiological Society",
number = "5",

}

TY - JOUR

T1 - Effect of claudins 6 and 9 on paracellular permeability in MDCK II cells

AU - Sas, David

AU - Hu, Mingchang

AU - Moe, Orson W.

AU - Baum, Michel

PY - 2008/11/1

Y1 - 2008/11/1

N2 - The neonatal proximal tubule has a lower permeability to chloride, higher resistance, and higher relative sodium-to-chloride permeability (P Na/PCl) than the adult tubule, which may be due to maturational changes in the tight junction. Claudins are tight-junction proteins between epithelial cells that determine paracellular permeability characteristics of epithelia. We have previously described the presence of two claudin isoforms, claudins 6 and 9, in the neonatal proximal tubule and subsequent reduction of these claudins during postnatal maturation. The question is whether changes in claudin expression are related to changes in functional characteristics in the neonatal tubule. We transfected claudins 6 and 9 into Madin-Darby canine kidney II (MDCK II) cells and performed electrophysiological studies to determine the resultant changes in physiological characteristics of the cells. Expression of claudins 6 and 9 resulted in an increased transepithelial resistance, decreased chloride permeability, and decreased PNa/PCl and PHCO3/PCl. These findings constitute the first characterization of the permeability characteristics of claudins 6 and 9 in a cell model and may explain why the neonatal proximal tubule has lower permeability to chloride and higher resistance than the adult proximal tubule.

AB - The neonatal proximal tubule has a lower permeability to chloride, higher resistance, and higher relative sodium-to-chloride permeability (P Na/PCl) than the adult tubule, which may be due to maturational changes in the tight junction. Claudins are tight-junction proteins between epithelial cells that determine paracellular permeability characteristics of epithelia. We have previously described the presence of two claudin isoforms, claudins 6 and 9, in the neonatal proximal tubule and subsequent reduction of these claudins during postnatal maturation. The question is whether changes in claudin expression are related to changes in functional characteristics in the neonatal tubule. We transfected claudins 6 and 9 into Madin-Darby canine kidney II (MDCK II) cells and performed electrophysiological studies to determine the resultant changes in physiological characteristics of the cells. Expression of claudins 6 and 9 resulted in an increased transepithelial resistance, decreased chloride permeability, and decreased PNa/PCl and PHCO3/PCl. These findings constitute the first characterization of the permeability characteristics of claudins 6 and 9 in a cell model and may explain why the neonatal proximal tubule has lower permeability to chloride and higher resistance than the adult proximal tubule.

KW - Neonate

KW - Proximal tubule

KW - Resistance

UR - http://www.scopus.com/inward/record.url?scp=57349141890&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57349141890&partnerID=8YFLogxK

U2 - 10.1152/ajpregu.90596.2008

DO - 10.1152/ajpregu.90596.2008

M3 - Article

C2 - 18784328

AN - SCOPUS:57349141890

VL - 295

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0363-6119

IS - 5

ER -