Effect of bacterial immunization on experimental reflux nephropathy

Vicente Torres, S. A. Kramer, K. E. Holley, G. W. Hartman, J. Bille, A. Ruiz-Arguelles, R. E. Ritts

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

This study was designed to test the hypothesis that young animals with vesicoureteral reflux might be more vulnerable to renal parenchymal infection by bacteria to which they had not been previously exposed. Forty-four crossbred male piglets had surgical induction of vesicoureteral reflux at 2 weeks of age and introduction of urinary tract infection at 6 weeks. They were sacrificed at 12 weeks of age. Between the ages 2 and 6 weeks, 22 piglets received subcutaneous injections of formalin-killed Escherichia coli in incomplete Freund's adjuvant as described. The remaining 22 piglets received incomplete Freund's adjuvant and vehicle alone. The antibody responses to antigenic challenge were weak to moderate. Immunized animals tended to have less renal scarring and better renal tubular uptake of dimercaptosuccinic acid, in addition to significantly lower serum creatinine values (p <0.001) and less mesangial cell proliferation in glomeruli (p = 0.05) We conclude that previous exposure to a specific bacterial immunization have at least a mild protective effect on the development of reflux nephropathy.

Original languageEnglish (US)
Pages (from-to)772-776
Number of pages5
JournalJournal of Urology
Volume131
Issue number4
StatePublished - 1984

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Immunization
Kidney
Succimer
Vesico-Ureteral Reflux
Mesangial Cells
Subcutaneous Injections
Urinary Tract Infections
Formaldehyde
Antibody Formation
Cicatrix
Creatinine
Cell Proliferation
Escherichia coli
Bacteria
Infection
Serum
incomplete Freund's adjuvant
Vesicoureteral Reflux 2

ASJC Scopus subject areas

  • Urology

Cite this

Torres, V., Kramer, S. A., Holley, K. E., Hartman, G. W., Bille, J., Ruiz-Arguelles, A., & Ritts, R. E. (1984). Effect of bacterial immunization on experimental reflux nephropathy. Journal of Urology, 131(4), 772-776.

Effect of bacterial immunization on experimental reflux nephropathy. / Torres, Vicente; Kramer, S. A.; Holley, K. E.; Hartman, G. W.; Bille, J.; Ruiz-Arguelles, A.; Ritts, R. E.

In: Journal of Urology, Vol. 131, No. 4, 1984, p. 772-776.

Research output: Contribution to journalArticle

Torres, V, Kramer, SA, Holley, KE, Hartman, GW, Bille, J, Ruiz-Arguelles, A & Ritts, RE 1984, 'Effect of bacterial immunization on experimental reflux nephropathy', Journal of Urology, vol. 131, no. 4, pp. 772-776.
Torres V, Kramer SA, Holley KE, Hartman GW, Bille J, Ruiz-Arguelles A et al. Effect of bacterial immunization on experimental reflux nephropathy. Journal of Urology. 1984;131(4):772-776.
Torres, Vicente ; Kramer, S. A. ; Holley, K. E. ; Hartman, G. W. ; Bille, J. ; Ruiz-Arguelles, A. ; Ritts, R. E. / Effect of bacterial immunization on experimental reflux nephropathy. In: Journal of Urology. 1984 ; Vol. 131, No. 4. pp. 772-776.
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AU - Ruiz-Arguelles, A.

AU - Ritts, R. E.

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N2 - This study was designed to test the hypothesis that young animals with vesicoureteral reflux might be more vulnerable to renal parenchymal infection by bacteria to which they had not been previously exposed. Forty-four crossbred male piglets had surgical induction of vesicoureteral reflux at 2 weeks of age and introduction of urinary tract infection at 6 weeks. They were sacrificed at 12 weeks of age. Between the ages 2 and 6 weeks, 22 piglets received subcutaneous injections of formalin-killed Escherichia coli in incomplete Freund's adjuvant as described. The remaining 22 piglets received incomplete Freund's adjuvant and vehicle alone. The antibody responses to antigenic challenge were weak to moderate. Immunized animals tended to have less renal scarring and better renal tubular uptake of dimercaptosuccinic acid, in addition to significantly lower serum creatinine values (p <0.001) and less mesangial cell proliferation in glomeruli (p = 0.05) We conclude that previous exposure to a specific bacterial immunization have at least a mild protective effect on the development of reflux nephropathy.

AB - This study was designed to test the hypothesis that young animals with vesicoureteral reflux might be more vulnerable to renal parenchymal infection by bacteria to which they had not been previously exposed. Forty-four crossbred male piglets had surgical induction of vesicoureteral reflux at 2 weeks of age and introduction of urinary tract infection at 6 weeks. They were sacrificed at 12 weeks of age. Between the ages 2 and 6 weeks, 22 piglets received subcutaneous injections of formalin-killed Escherichia coli in incomplete Freund's adjuvant as described. The remaining 22 piglets received incomplete Freund's adjuvant and vehicle alone. The antibody responses to antigenic challenge were weak to moderate. Immunized animals tended to have less renal scarring and better renal tubular uptake of dimercaptosuccinic acid, in addition to significantly lower serum creatinine values (p <0.001) and less mesangial cell proliferation in glomeruli (p = 0.05) We conclude that previous exposure to a specific bacterial immunization have at least a mild protective effect on the development of reflux nephropathy.

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