Abstract
To investigate the role of insulin-like growth factor II in the control of DNA synthesis in human fibroblasts, dose-response curves for insulin-like growth factor I and II stimulation of [3H]thymidine incorporation were compared in the absence and presence of alphaIR-3, a highly specific monoclonal antibody directed against the type I insulin-like growth factor receptor. Specific binding of [125I]insulin-like growth factor I to human fibroblast monolayer cultures was inhibited 60-70% in the presence of alphaIR-3. alphaIR-3 had no effect on [125I]insulin-like growth factor II binding to human fibroblasts. However, alphaIR-3 inhibited both insulin-like growth factor I and II stimulated [3H]thymidine incorporation. These data indicate that the type II insulin-like growth factor receptor does not function as a transducer of insulin-like growth factor II's mitogenic effect in human fibroblasts.
Original language | English (US) |
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Pages (from-to) | 501-508 |
Number of pages | 8 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 139 |
Issue number | 2 |
DOIs | |
State | Published - Sep 16 1986 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology