Effect of a model of canine jejunoileal orthotopic autotransplantation on jejunal and ileal transport of water and electrolytes

A. M. Walters, A. R. Zinsmeister, M. G. Sarr

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Canine jejunoileal transplantation induces an early profuse watery diarrhea of uncertain etiology. Our aim was to determine the temporal effects of a canine model of jejunoileal autotransplantation (a model devoid of confounding effects of ischemia-reperfusion or immune rejection) on basal jejunal and ileal absorption of water and electrolytes to determine if impaired absorption is responsible for the diarrhea. Our hypothesis was that net absorption of water and electrolytes in an enterically isolated loop would decrease after jejunoileal transplantation. Four groups of dogs (N ≥ 6) were prepared with 80-cm modified Thiry-Vella loops: group I, neurally intact jejunum; group II, autotransplanted jejunum; group III, neurally intact ileum; and group IV, autotransplanted ileum. The loops were perfused for 3 hr with 150 mM NaCI at 3 ml/min under fasted conditions; transit time through the loop was determined by bolus of a nonabsorbable marker. Dogs were studied on three separate days at one, two, eight, and nine weeks postoperatively. Net absorptive fluxes of water and electrolytes and transit times were similar (P > 0.05) between neurally intact and autotransplant groups (group I vs II and group III vs IV at each time point. Ileal loops absorbed more than jejunal loops, and transit was slower in ileal loops (each P < 0.05). Our findings suggest that, despite the obligate disruption of extrinsic innervation, enteric (intrinsic) neural continuity, and Iymphatic drainage that accompanies this canine model of jejunoileal autotransplantation, net basal absorptive function of water and electrolytes during the fasted state was not decreased nor was transit altered either in jejunum or ileum. These findings have important implications for clinical small intestinal transplantation in man.

Original languageEnglish (US)
Pages (from-to)843-850
Number of pages8
JournalDigestive Diseases and Sciences
Volume39
Issue number4
StatePublished - 1994

Fingerprint

Autologous Transplantation
Electrolytes
Canidae
Jejunum
Ileum
Water
Transplantation
Diarrhea
Dogs
Autografts
Reperfusion
Drainage
Ischemia

Keywords

  • Absorption
  • Intestinal transit
  • Intestinal transplantation
  • Jejunoileal autotransplantation

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Effect of a model of canine jejunoileal orthotopic autotransplantation on jejunal and ileal transport of water and electrolytes. / Walters, A. M.; Zinsmeister, A. R.; Sarr, M. G.

In: Digestive Diseases and Sciences, Vol. 39, No. 4, 1994, p. 843-850.

Research output: Contribution to journalArticle

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