Effect of 3,5,3'-triiodothyronine and 3,3',5'-triiodothyronine administration on serum-tri, di-, and monoiodothyronines and plasma cyclic nucleotides in sheep

H. L. Wray, K. D. Burman, Robert Christian Smallridge, J. P. Alford, V. M. Butler, F. D. Wright, L. Wartofsky

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

T 3 and/or rT 3 were administered to sheep in order 1) to determine their influence on plasma cyclic nucleotides and 2) to examine their effects on serum T 4, T 3, rT 3, 3',5'-diiodothyronine (3',5'T 2), 3,3'T 2, and 3'-monoiodothyronine (3'T 1). The animals were divided into five groups, and each group received one of the following treatments: 1) placebo, 2) low T 3 (1.5 μg/kg), 3) high T 3 (4.5 μg/kg), 4) high rT 3 (4 μg/kg), or 5) low rT 3 (2.5 μg/kg) plus low T 3 (1.5 μg/kg) in combination. Treatments were administered every 8 h for 6 days, and the animals were studied throughout this period. Cyclic nucleotides and iodothyronines were measured by RIA. High dose T 3 treatment increased plasma cAMP by 52% (P < 0.02), whereas plasma cGMP was unaffected. Other treatments did not alter plasma cyclic nucleotides. Both low and high dose T 3 treatment decreased T 4 and rT 3 levels, whereas rT 3 treatment did not alter levels of serum T 4 or T 3. Serum 3,3'T 2 concentrations were increased by rT 3 and T 3 treatment, both singly and in combination. Serum 3',5'T 2 levels were undetectable (< 0.055 nM), except after rT 3 administration. 3'T 1 levels were unchanged by treatments, except that high T 3 administration slightly, but significantly, decreased serum 3'T 1 concentrations. These results indicate that in the sheep 1) T 3 markedly increases plasma cAMP and decreases T 4 and rT 3 levels, whereas rT 3 administration is inactive in both of these systems; 2) neither T 3 nor rT 3 alters plasma cGMP; 3) degradation of rT 3 is directed relatively more to 3,3'T 2 than to 3',5'T 2; 4) metabolism of both T 3 and rT 3 contribute comparably to 3,3'T 2 levels; and 5) T 3 may enhance the conversion of rT 3 to both 3,3'T 2 and 3',5T 2.

Original languageEnglish (US)
Pages (from-to)130-136
Number of pages7
JournalEndocrinology
Volume107
Issue number1
StatePublished - 1980
Externally publishedYes

Fingerprint

Diiodothyronines
Reverse Triiodothyronine
Cyclic Nucleotides
Triiodothyronine
Sheep
Serum
Therapeutics
Placebos

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Effect of 3,5,3'-triiodothyronine and 3,3',5'-triiodothyronine administration on serum-tri, di-, and monoiodothyronines and plasma cyclic nucleotides in sheep. / Wray, H. L.; Burman, K. D.; Smallridge, Robert Christian; Alford, J. P.; Butler, V. M.; Wright, F. D.; Wartofsky, L.

In: Endocrinology, Vol. 107, No. 1, 1980, p. 130-136.

Research output: Contribution to journalArticle

Wray, H. L. ; Burman, K. D. ; Smallridge, Robert Christian ; Alford, J. P. ; Butler, V. M. ; Wright, F. D. ; Wartofsky, L. / Effect of 3,5,3'-triiodothyronine and 3,3',5'-triiodothyronine administration on serum-tri, di-, and monoiodothyronines and plasma cyclic nucleotides in sheep. In: Endocrinology. 1980 ; Vol. 107, No. 1. pp. 130-136.
@article{021ca293abea47b58369dd712b49972a,
title = "Effect of 3,5,3'-triiodothyronine and 3,3',5'-triiodothyronine administration on serum-tri, di-, and monoiodothyronines and plasma cyclic nucleotides in sheep",
abstract = "T 3 and/or rT 3 were administered to sheep in order 1) to determine their influence on plasma cyclic nucleotides and 2) to examine their effects on serum T 4, T 3, rT 3, 3',5'-diiodothyronine (3',5'T 2), 3,3'T 2, and 3'-monoiodothyronine (3'T 1). The animals were divided into five groups, and each group received one of the following treatments: 1) placebo, 2) low T 3 (1.5 μg/kg), 3) high T 3 (4.5 μg/kg), 4) high rT 3 (4 μg/kg), or 5) low rT 3 (2.5 μg/kg) plus low T 3 (1.5 μg/kg) in combination. Treatments were administered every 8 h for 6 days, and the animals were studied throughout this period. Cyclic nucleotides and iodothyronines were measured by RIA. High dose T 3 treatment increased plasma cAMP by 52{\%} (P < 0.02), whereas plasma cGMP was unaffected. Other treatments did not alter plasma cyclic nucleotides. Both low and high dose T 3 treatment decreased T 4 and rT 3 levels, whereas rT 3 treatment did not alter levels of serum T 4 or T 3. Serum 3,3'T 2 concentrations were increased by rT 3 and T 3 treatment, both singly and in combination. Serum 3',5'T 2 levels were undetectable (< 0.055 nM), except after rT 3 administration. 3'T 1 levels were unchanged by treatments, except that high T 3 administration slightly, but significantly, decreased serum 3'T 1 concentrations. These results indicate that in the sheep 1) T 3 markedly increases plasma cAMP and decreases T 4 and rT 3 levels, whereas rT 3 administration is inactive in both of these systems; 2) neither T 3 nor rT 3 alters plasma cGMP; 3) degradation of rT 3 is directed relatively more to 3,3'T 2 than to 3',5'T 2; 4) metabolism of both T 3 and rT 3 contribute comparably to 3,3'T 2 levels; and 5) T 3 may enhance the conversion of rT 3 to both 3,3'T 2 and 3',5T 2.",
author = "Wray, {H. L.} and Burman, {K. D.} and Smallridge, {Robert Christian} and Alford, {J. P.} and Butler, {V. M.} and Wright, {F. D.} and L. Wartofsky",
year = "1980",
language = "English (US)",
volume = "107",
pages = "130--136",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "1",

}

TY - JOUR

T1 - Effect of 3,5,3'-triiodothyronine and 3,3',5'-triiodothyronine administration on serum-tri, di-, and monoiodothyronines and plasma cyclic nucleotides in sheep

AU - Wray, H. L.

AU - Burman, K. D.

AU - Smallridge, Robert Christian

AU - Alford, J. P.

AU - Butler, V. M.

AU - Wright, F. D.

AU - Wartofsky, L.

PY - 1980

Y1 - 1980

N2 - T 3 and/or rT 3 were administered to sheep in order 1) to determine their influence on plasma cyclic nucleotides and 2) to examine their effects on serum T 4, T 3, rT 3, 3',5'-diiodothyronine (3',5'T 2), 3,3'T 2, and 3'-monoiodothyronine (3'T 1). The animals were divided into five groups, and each group received one of the following treatments: 1) placebo, 2) low T 3 (1.5 μg/kg), 3) high T 3 (4.5 μg/kg), 4) high rT 3 (4 μg/kg), or 5) low rT 3 (2.5 μg/kg) plus low T 3 (1.5 μg/kg) in combination. Treatments were administered every 8 h for 6 days, and the animals were studied throughout this period. Cyclic nucleotides and iodothyronines were measured by RIA. High dose T 3 treatment increased plasma cAMP by 52% (P < 0.02), whereas plasma cGMP was unaffected. Other treatments did not alter plasma cyclic nucleotides. Both low and high dose T 3 treatment decreased T 4 and rT 3 levels, whereas rT 3 treatment did not alter levels of serum T 4 or T 3. Serum 3,3'T 2 concentrations were increased by rT 3 and T 3 treatment, both singly and in combination. Serum 3',5'T 2 levels were undetectable (< 0.055 nM), except after rT 3 administration. 3'T 1 levels were unchanged by treatments, except that high T 3 administration slightly, but significantly, decreased serum 3'T 1 concentrations. These results indicate that in the sheep 1) T 3 markedly increases plasma cAMP and decreases T 4 and rT 3 levels, whereas rT 3 administration is inactive in both of these systems; 2) neither T 3 nor rT 3 alters plasma cGMP; 3) degradation of rT 3 is directed relatively more to 3,3'T 2 than to 3',5'T 2; 4) metabolism of both T 3 and rT 3 contribute comparably to 3,3'T 2 levels; and 5) T 3 may enhance the conversion of rT 3 to both 3,3'T 2 and 3',5T 2.

AB - T 3 and/or rT 3 were administered to sheep in order 1) to determine their influence on plasma cyclic nucleotides and 2) to examine their effects on serum T 4, T 3, rT 3, 3',5'-diiodothyronine (3',5'T 2), 3,3'T 2, and 3'-monoiodothyronine (3'T 1). The animals were divided into five groups, and each group received one of the following treatments: 1) placebo, 2) low T 3 (1.5 μg/kg), 3) high T 3 (4.5 μg/kg), 4) high rT 3 (4 μg/kg), or 5) low rT 3 (2.5 μg/kg) plus low T 3 (1.5 μg/kg) in combination. Treatments were administered every 8 h for 6 days, and the animals were studied throughout this period. Cyclic nucleotides and iodothyronines were measured by RIA. High dose T 3 treatment increased plasma cAMP by 52% (P < 0.02), whereas plasma cGMP was unaffected. Other treatments did not alter plasma cyclic nucleotides. Both low and high dose T 3 treatment decreased T 4 and rT 3 levels, whereas rT 3 treatment did not alter levels of serum T 4 or T 3. Serum 3,3'T 2 concentrations were increased by rT 3 and T 3 treatment, both singly and in combination. Serum 3',5'T 2 levels were undetectable (< 0.055 nM), except after rT 3 administration. 3'T 1 levels were unchanged by treatments, except that high T 3 administration slightly, but significantly, decreased serum 3'T 1 concentrations. These results indicate that in the sheep 1) T 3 markedly increases plasma cAMP and decreases T 4 and rT 3 levels, whereas rT 3 administration is inactive in both of these systems; 2) neither T 3 nor rT 3 alters plasma cGMP; 3) degradation of rT 3 is directed relatively more to 3,3'T 2 than to 3',5'T 2; 4) metabolism of both T 3 and rT 3 contribute comparably to 3,3'T 2 levels; and 5) T 3 may enhance the conversion of rT 3 to both 3,3'T 2 and 3',5T 2.

UR - http://www.scopus.com/inward/record.url?scp=0018855653&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018855653&partnerID=8YFLogxK

M3 - Article

C2 - 6247133

AN - SCOPUS:0018855653

VL - 107

SP - 130

EP - 136

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 1

ER -