Abstract
The aim of this manuscript and accompanying video is to provide an overview of the methods and approaches used for imaging heart valve function in rodents, with detailed descriptions of the appropriate methods for anesthesia, the echocardiographic windows used, the imaging planes and probe orientations for image acquisition, the methods for data analysis, and the limitations of emerging technologies for the evaluation of cardiac and valvular function. Importantly, we also highlight several future areas of research in cardiac and heart valve imaging that may be leveraged to gain insights into the pathogenesis of valve disease in preclinical animal models. We propose that using a systematic approach to evaluating cardiac and heart valve function in mice can result in more robust and reproducible data, as well as facilitate the discovery of previously underappreciated phenotypes in genetically-altered and/or physiologically-stressed mice.
Original language | English (US) |
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Article number | e54110 |
Journal | Journal of Visualized Experiments |
Volume | 2017 |
Issue number | 120 |
DOIs | |
State | Published - Feb 14 2017 |
Keywords
- 2D-echocardiogram
- Animal cardiac imaging
- Aortic valve
- Aortic valve stenosis
- Cardiac function
- Doppler
- Isoflurane
- Issue 120
- Medicine
- Strain and strain rate imaging
- Tissue doppler
ASJC Scopus subject areas
- General Neuroscience
- General Chemical Engineering
- General Biochemistry, Genetics and Molecular Biology
- General Immunology and Microbiology