Ebola Virus Matrix Protein VP40 Uses the COPII Transport System for Its Intracellular Transport

Seiya Yamayoshi, Takeshi Noda, Hideki Ebihara, Hideo Goto, Yuko Morikawa, Igor S. Lukashevich, Gabriele Neumann, Heinz Feldmann, Yoshihiro Kawaoka

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59 Scopus citations

Abstract

The Ebola virus matrix protein VP40 plays an important role in virion formation and viral egress from cells. However, the host cell proteins and mechanisms responsible for intracellular transport of VP40 prior to its contribution to virion formation remain to be elucidated. Therefore we used coimmunoprecipitation and mass spectrometric analyses to identify host proteins interacting with VP40. We found that Sec24C, a component of the host COPII vesicular transport system, interacts specifically with VP40 via VP40 amino acids 303 to 307. Coimmunoprecipitation and dominant-negative mutant studies indicated that the COPII transport system plays a critical role in VP40 intracellular transport to the plasma membrane. Marburg virus VP40 was also shown to use the COPII transport system for intracellular transport. These findings identify a conserved intersection between a host pathway and filovirus replication, an intersection that can be targeted in the development of new antiviral drugs.

Original languageEnglish (US)
Pages (from-to)168-177
Number of pages10
JournalCell Host and Microbe
Volume3
Issue number3
DOIs
StatePublished - Mar 13 2008

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Keywords

  • CELLBIO
  • MICROBIO

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology

Cite this

Yamayoshi, S., Noda, T., Ebihara, H., Goto, H., Morikawa, Y., Lukashevich, I. S., Neumann, G., Feldmann, H., & Kawaoka, Y. (2008). Ebola Virus Matrix Protein VP40 Uses the COPII Transport System for Its Intracellular Transport. Cell Host and Microbe, 3(3), 168-177. https://doi.org/10.1016/j.chom.2008.02.001