Ebola virus glycoprotein-mediated anoikis of primary human cardiac microvascular endothelial cells

Ratna B. Ray, Arnab Basu, Robert Steele, Aster Beyene, Jane McHowat, Keith Meyer, Asish K. Ghosh, Ranjit Ray

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Ebola virus glycoprotein (EGP) has been implicated for the induction of cytotoxicity and injury in vascular cells. On the other hand, EGP has also been suggested to induce massive cell rounding and detachment from the plastic surface by downregulating cell adhesion molecules without causing cytotoxicity. In this study, we have examined the cytotoxic role of EGP in primary endothelial cells by transduction with a replication-deficient recombinant adenovirus expressing EGP (Ad-EGP). Primary human cardiac microvascular endothelial cells (HCMECs) transduced with Ad-EGP displayed loss of cell adhesion from the plastic surface followed by cell death. Transfer of conditioned medium from EGP-transduced HCMEC into naive cells did not induce loss of adhesion or cell death, suggesting that EGP needs to be expressed intracellularly to exert its cytotoxic effect. Subsequent studies suggested that HCMEC death occurred through apoptosis. Results from this study shed light on the EGP-induced anoikis in primary human cardiac endothelial cells, which may have significant pathological consequences.

Original languageEnglish (US)
Pages (from-to)181-188
Number of pages8
JournalVirology
Volume321
Issue number2
DOIs
StatePublished - Apr 10 2004
Externally publishedYes

Fingerprint

Anoikis
Ebolavirus
Glycoproteins
Endothelial Cells
Cell Death
Adenoviridae
Plastics
Vascular System Injuries
Cell Adhesion Molecules
Conditioned Culture Medium
Cell Adhesion
Down-Regulation
Apoptosis

Keywords

  • Anoikis
  • Ebola virus glycoprotein
  • Human cardiac microvascular endothelial cell

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Ray, R. B., Basu, A., Steele, R., Beyene, A., McHowat, J., Meyer, K., ... Ray, R. (2004). Ebola virus glycoprotein-mediated anoikis of primary human cardiac microvascular endothelial cells. Virology, 321(2), 181-188. https://doi.org/10.1016/j.virol.2003.12.014

Ebola virus glycoprotein-mediated anoikis of primary human cardiac microvascular endothelial cells. / Ray, Ratna B.; Basu, Arnab; Steele, Robert; Beyene, Aster; McHowat, Jane; Meyer, Keith; Ghosh, Asish K.; Ray, Ranjit.

In: Virology, Vol. 321, No. 2, 10.04.2004, p. 181-188.

Research output: Contribution to journalArticle

Ray, RB, Basu, A, Steele, R, Beyene, A, McHowat, J, Meyer, K, Ghosh, AK & Ray, R 2004, 'Ebola virus glycoprotein-mediated anoikis of primary human cardiac microvascular endothelial cells', Virology, vol. 321, no. 2, pp. 181-188. https://doi.org/10.1016/j.virol.2003.12.014
Ray, Ratna B. ; Basu, Arnab ; Steele, Robert ; Beyene, Aster ; McHowat, Jane ; Meyer, Keith ; Ghosh, Asish K. ; Ray, Ranjit. / Ebola virus glycoprotein-mediated anoikis of primary human cardiac microvascular endothelial cells. In: Virology. 2004 ; Vol. 321, No. 2. pp. 181-188.
@article{35b856c62e17411b919cbde6f4c650d6,
title = "Ebola virus glycoprotein-mediated anoikis of primary human cardiac microvascular endothelial cells",
abstract = "Ebola virus glycoprotein (EGP) has been implicated for the induction of cytotoxicity and injury in vascular cells. On the other hand, EGP has also been suggested to induce massive cell rounding and detachment from the plastic surface by downregulating cell adhesion molecules without causing cytotoxicity. In this study, we have examined the cytotoxic role of EGP in primary endothelial cells by transduction with a replication-deficient recombinant adenovirus expressing EGP (Ad-EGP). Primary human cardiac microvascular endothelial cells (HCMECs) transduced with Ad-EGP displayed loss of cell adhesion from the plastic surface followed by cell death. Transfer of conditioned medium from EGP-transduced HCMEC into naive cells did not induce loss of adhesion or cell death, suggesting that EGP needs to be expressed intracellularly to exert its cytotoxic effect. Subsequent studies suggested that HCMEC death occurred through apoptosis. Results from this study shed light on the EGP-induced anoikis in primary human cardiac endothelial cells, which may have significant pathological consequences.",
keywords = "Anoikis, Ebola virus glycoprotein, Human cardiac microvascular endothelial cell",
author = "Ray, {Ratna B.} and Arnab Basu and Robert Steele and Aster Beyene and Jane McHowat and Keith Meyer and Ghosh, {Asish K.} and Ranjit Ray",
year = "2004",
month = "4",
day = "10",
doi = "10.1016/j.virol.2003.12.014",
language = "English (US)",
volume = "321",
pages = "181--188",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Ebola virus glycoprotein-mediated anoikis of primary human cardiac microvascular endothelial cells

AU - Ray, Ratna B.

AU - Basu, Arnab

AU - Steele, Robert

AU - Beyene, Aster

AU - McHowat, Jane

AU - Meyer, Keith

AU - Ghosh, Asish K.

AU - Ray, Ranjit

PY - 2004/4/10

Y1 - 2004/4/10

N2 - Ebola virus glycoprotein (EGP) has been implicated for the induction of cytotoxicity and injury in vascular cells. On the other hand, EGP has also been suggested to induce massive cell rounding and detachment from the plastic surface by downregulating cell adhesion molecules without causing cytotoxicity. In this study, we have examined the cytotoxic role of EGP in primary endothelial cells by transduction with a replication-deficient recombinant adenovirus expressing EGP (Ad-EGP). Primary human cardiac microvascular endothelial cells (HCMECs) transduced with Ad-EGP displayed loss of cell adhesion from the plastic surface followed by cell death. Transfer of conditioned medium from EGP-transduced HCMEC into naive cells did not induce loss of adhesion or cell death, suggesting that EGP needs to be expressed intracellularly to exert its cytotoxic effect. Subsequent studies suggested that HCMEC death occurred through apoptosis. Results from this study shed light on the EGP-induced anoikis in primary human cardiac endothelial cells, which may have significant pathological consequences.

AB - Ebola virus glycoprotein (EGP) has been implicated for the induction of cytotoxicity and injury in vascular cells. On the other hand, EGP has also been suggested to induce massive cell rounding and detachment from the plastic surface by downregulating cell adhesion molecules without causing cytotoxicity. In this study, we have examined the cytotoxic role of EGP in primary endothelial cells by transduction with a replication-deficient recombinant adenovirus expressing EGP (Ad-EGP). Primary human cardiac microvascular endothelial cells (HCMECs) transduced with Ad-EGP displayed loss of cell adhesion from the plastic surface followed by cell death. Transfer of conditioned medium from EGP-transduced HCMEC into naive cells did not induce loss of adhesion or cell death, suggesting that EGP needs to be expressed intracellularly to exert its cytotoxic effect. Subsequent studies suggested that HCMEC death occurred through apoptosis. Results from this study shed light on the EGP-induced anoikis in primary human cardiac endothelial cells, which may have significant pathological consequences.

KW - Anoikis

KW - Ebola virus glycoprotein

KW - Human cardiac microvascular endothelial cell

UR - http://www.scopus.com/inward/record.url?scp=1642446620&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1642446620&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2003.12.014

DO - 10.1016/j.virol.2003.12.014

M3 - Article

C2 - 15051379

AN - SCOPUS:1642446620

VL - 321

SP - 181

EP - 188

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

ER -