Early phase bronchoconstriction in the mouse requires allergen-specific IgG

Jeffrey R. Crosby, Grzegorz Cieslewicz, Michael Borchers, Edie Hines, Patricia Carrigan, James J. Lee, Nancy A Lee

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Allergen provocation of allergic asthma patients is often characterized by an initial period of bronchoconstriction, or early phase reaction (EPR), that leads to maximal airway narrowing within 15-30 min, followed by a recovery period returning airway function to baseline within 1-2 h. In this study, we used a defined OVA provocation model and mice deficient for specific leukocyte populations to investigate the cellular/molecular origins of the EPR. OVA-sensitized/challenged wild-type (C57BL/6J) mice displayed an EPR following OVA provocation. However, this response was absent in gene knockout animals deficient of either B or T cells. Moreover, transfer of OVA-specific IgG, but not IgE, before the OVA provocation, was capable of inducing the EPR in both strains of lymphocyte-deficient mice. Interestingly, an EPR was also observed in sensitized/challenged mast cell-deficient mice following an OVA provocation. These data show that the EPR in the mouse is an immunologically based pathophysiological response that requires allergen-specific IgG but occurs independent of mast cell activities. Thus, in the mouse the initial period of bronchoconstriction following allergen exposure may involve neither mast cells nor IgE-mediated events.

Original languageEnglish (US)
Pages (from-to)4050-4054
Number of pages5
JournalJournal of Immunology
Volume168
Issue number8
StatePublished - Apr 15 2002

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Bronchoconstriction
Allergens
Immunoglobulin G
Mast Cells
Immunoglobulin E
Gene Knockout Techniques
Inbred C57BL Mouse
Leukocytes
B-Lymphocytes
Asthma
Lymphocytes
T-Lymphocytes
Population

ASJC Scopus subject areas

  • Immunology

Cite this

Crosby, J. R., Cieslewicz, G., Borchers, M., Hines, E., Carrigan, P., Lee, J. J., & Lee, N. A. (2002). Early phase bronchoconstriction in the mouse requires allergen-specific IgG. Journal of Immunology, 168(8), 4050-4054.

Early phase bronchoconstriction in the mouse requires allergen-specific IgG. / Crosby, Jeffrey R.; Cieslewicz, Grzegorz; Borchers, Michael; Hines, Edie; Carrigan, Patricia; Lee, James J.; Lee, Nancy A.

In: Journal of Immunology, Vol. 168, No. 8, 15.04.2002, p. 4050-4054.

Research output: Contribution to journalArticle

Crosby, JR, Cieslewicz, G, Borchers, M, Hines, E, Carrigan, P, Lee, JJ & Lee, NA 2002, 'Early phase bronchoconstriction in the mouse requires allergen-specific IgG', Journal of Immunology, vol. 168, no. 8, pp. 4050-4054.
Crosby JR, Cieslewicz G, Borchers M, Hines E, Carrigan P, Lee JJ et al. Early phase bronchoconstriction in the mouse requires allergen-specific IgG. Journal of Immunology. 2002 Apr 15;168(8):4050-4054.
Crosby, Jeffrey R. ; Cieslewicz, Grzegorz ; Borchers, Michael ; Hines, Edie ; Carrigan, Patricia ; Lee, James J. ; Lee, Nancy A. / Early phase bronchoconstriction in the mouse requires allergen-specific IgG. In: Journal of Immunology. 2002 ; Vol. 168, No. 8. pp. 4050-4054.
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