Early induction of neuronal lipocalin-type prostaglandin D synthase after hypoxic-ischemic injury in developing brains

Hidetoshi Taniguchi, Ikuko Mohri, Hitomi Okabe-Arahori, Takahisa Kanekiyo, Kuriko Kagitani-Shimono, Kazuko Wada, Yoshihiro Urade, Masahiro Nakayama, Keiichi Ozono, Masako Taniike

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Abstract

Lipocalin-type prostaglandin (PG) D synthase (L-PGDS) is up-regulated in oligodendrocytes (OLs) in mouse models for genetic neurological disorders including globoid cell leukodystrophy (twitcher) and GM1 and GM2 gangliosidoses and in the brain of patients with multiple sclerosis. Since L-PGDS-deficient twitcher mice undergo extensive neuronal death, we concluded that L-PGDS functions protectively against neuronal degeneration. In this study, we investigated whether L-PGDS is also up-regulated in acute and massive brain injury resulting from neonatal hypoxic-ischemic encephalopathy (HIE). Analysis of brains from human neonates who had died from HIE disclosed that the surviving neurons in the infarcted lesions expressed L-PGDS. Mouse models for neonatal HIE were made on postnatal day (PND) 7. Global infarction in the ipsilateral hemisphere was evident at 24 h after reoxygenation in this model. Intense L-PGDS immunoreactivity was already observed at 10 min after reoxygenation in apparently normal neurons in the cortex, and thereafter, in neurons adjacent to the infarcted area. Quantitative RT-PCR revealed that the L-PGDS mRNA level of the infarcted hemisphere was 33-fold higher than that of the sham-operated mouse brains at 1 h after reoxygenation and that it decreased to the normal level by 24 h thereafter. Furthermore, in both human and mouse brains, many of L-PGDS-positive cells were also immunoreactive for p53; and some of these expressed cleaved caspase-3. The expression of L-PGDS in degenerating neurons implies that L-PGDS functions as an early stress protein to protect against neuronal death in the HIE brain.

Original languageEnglish (US)
Pages (from-to)39-44
Number of pages6
JournalNeuroscience Letters
Volume420
Issue number1
DOIs
StatePublished - Jun 8 2007
Externally publishedYes

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prostaglandin R2 D-isomerase
Lipocalins
Brain Hypoxia-Ischemia
Wounds and Injuries
Brain
Neurons
GM2 Gangliosidosis
GM1 Gangliosidosis
Globoid Cell Leukodystrophy
Inborn Genetic Diseases
Oligodendroglia
Heat-Shock Proteins
Nervous System Diseases
Caspase 3
Brain Injuries
Infarction
Multiple Sclerosis
Newborn Infant
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Apoptosis
  • Hypoxic-ischemic encephalopathy
  • Lipocalin-type prostaglandin D synthase
  • Neonate
  • Prostaglandin D

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Early induction of neuronal lipocalin-type prostaglandin D synthase after hypoxic-ischemic injury in developing brains. / Taniguchi, Hidetoshi; Mohri, Ikuko; Okabe-Arahori, Hitomi; Kanekiyo, Takahisa; Kagitani-Shimono, Kuriko; Wada, Kazuko; Urade, Yoshihiro; Nakayama, Masahiro; Ozono, Keiichi; Taniike, Masako.

In: Neuroscience Letters, Vol. 420, No. 1, 08.06.2007, p. 39-44.

Research output: Contribution to journalArticle

Taniguchi, H, Mohri, I, Okabe-Arahori, H, Kanekiyo, T, Kagitani-Shimono, K, Wada, K, Urade, Y, Nakayama, M, Ozono, K & Taniike, M 2007, 'Early induction of neuronal lipocalin-type prostaglandin D synthase after hypoxic-ischemic injury in developing brains', Neuroscience Letters, vol. 420, no. 1, pp. 39-44. https://doi.org/10.1016/j.neulet.2007.04.016
Taniguchi, Hidetoshi ; Mohri, Ikuko ; Okabe-Arahori, Hitomi ; Kanekiyo, Takahisa ; Kagitani-Shimono, Kuriko ; Wada, Kazuko ; Urade, Yoshihiro ; Nakayama, Masahiro ; Ozono, Keiichi ; Taniike, Masako. / Early induction of neuronal lipocalin-type prostaglandin D synthase after hypoxic-ischemic injury in developing brains. In: Neuroscience Letters. 2007 ; Vol. 420, No. 1. pp. 39-44.
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