Dysregulated microRNAs and their predicted targets associated with endometrioid endometrial adenocarcinoma in Hong Kong women

Tony K H Chung, Tak Hong Cheung, Ngar Yee Huen, Katherine W Y Wong, Keith W K Lo, So Fan Yim, Nelson S S Siu, Yin Mei Wong, Po Ting Tsang, Man Wah Pang, Mei Yun Yu, Ka Fei To, Samuel C. Mok, Vivian W. Wang, Chen Li, Albert Y K Cheung, Graeme Doran, Michael J. Birrer, David I Smith, Yick Fu Wong

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

The objective of this study, a parallel study to global gene expression profiling, was to identify dysregulated microRNAs (miRNAs) associated with endometrioid endometrial adenocarcinoma (EEC), examine their correlation with clinico-pathoiogicai characteristics and identify predicted target genes of the dysregulated miRNAs. Using real-time quantitative reverse transcription-poiy- merase chain reaction (qRT-PCR), profiling of miRNA expression was performed in 30 EECs and 22 normal counterparts in which genome-wide gene expression had been previously profiled and reported. Clustering analysis identified 30 miRNAs which were significantly dysregulated in EEC. The expression of a sub-group of miRNAs was significantly correlated with clinico-pathological characteristics including stage, myometrial invasion, recurrence and lymph node involvement. By searching for predicted miRNA targets that were linked to the dysregulated genes previously identified, 68 genes were predicted as candidate targets of these 30 dysregulated miRNAs. miR-205 was significantly overexpressed in EECs compared with normal controls. After transfection of a miR-205 inhibitor, the expression of miR-205 in endometrial cancer cell line RL95-2 cells decreased whereas its predicted target gene, JPH.4, showed increased protein expression. JPH4 seems to be a real miR-205 target in vitro and in vivo, and a candidate tumor suppressor gene in EEC. Based on this study in EEC, miRNAs predicted to be involved in tumorigenesis and tumor progression have been identified and placed in the context of the transcriptome of EEC. This work provides a framework on which further research into novel diagnosis and treatment of EEC can be focused.

Original languageEnglish (US)
Pages (from-to)1358-1365
Number of pages8
JournalInternational Journal of Cancer
Volume124
Issue number6
DOIs
StatePublished - Mar 15 2009

Fingerprint

Endometrioid Carcinoma
Hong Kong
MicroRNAs
European Union
Genes
Gene Expression Profiling
Endometrial Neoplasms
Tumor Suppressor Genes
Transcriptome
Reverse Transcription
Transfection
Cluster Analysis
Carcinogenesis
Lymph Nodes
Genome
Gene Expression
Recurrence
Cell Line

Keywords

  • Adenocarcinoma
  • Endometrial
  • MicroRNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Medicine(all)

Cite this

Chung, T. K. H., Cheung, T. H., Huen, N. Y., Wong, K. W. Y., Lo, K. W. K., Yim, S. F., ... Wong, Y. F. (2009). Dysregulated microRNAs and their predicted targets associated with endometrioid endometrial adenocarcinoma in Hong Kong women. International Journal of Cancer, 124(6), 1358-1365. https://doi.org/10.1002/ijc.24071

Dysregulated microRNAs and their predicted targets associated with endometrioid endometrial adenocarcinoma in Hong Kong women. / Chung, Tony K H; Cheung, Tak Hong; Huen, Ngar Yee; Wong, Katherine W Y; Lo, Keith W K; Yim, So Fan; Siu, Nelson S S; Wong, Yin Mei; Tsang, Po Ting; Pang, Man Wah; Yu, Mei Yun; To, Ka Fei; Mok, Samuel C.; Wang, Vivian W.; Li, Chen; Cheung, Albert Y K; Doran, Graeme; Birrer, Michael J.; Smith, David I; Wong, Yick Fu.

In: International Journal of Cancer, Vol. 124, No. 6, 15.03.2009, p. 1358-1365.

Research output: Contribution to journalArticle

Chung, TKH, Cheung, TH, Huen, NY, Wong, KWY, Lo, KWK, Yim, SF, Siu, NSS, Wong, YM, Tsang, PT, Pang, MW, Yu, MY, To, KF, Mok, SC, Wang, VW, Li, C, Cheung, AYK, Doran, G, Birrer, MJ, Smith, DI & Wong, YF 2009, 'Dysregulated microRNAs and their predicted targets associated with endometrioid endometrial adenocarcinoma in Hong Kong women', International Journal of Cancer, vol. 124, no. 6, pp. 1358-1365. https://doi.org/10.1002/ijc.24071
Chung, Tony K H ; Cheung, Tak Hong ; Huen, Ngar Yee ; Wong, Katherine W Y ; Lo, Keith W K ; Yim, So Fan ; Siu, Nelson S S ; Wong, Yin Mei ; Tsang, Po Ting ; Pang, Man Wah ; Yu, Mei Yun ; To, Ka Fei ; Mok, Samuel C. ; Wang, Vivian W. ; Li, Chen ; Cheung, Albert Y K ; Doran, Graeme ; Birrer, Michael J. ; Smith, David I ; Wong, Yick Fu. / Dysregulated microRNAs and their predicted targets associated with endometrioid endometrial adenocarcinoma in Hong Kong women. In: International Journal of Cancer. 2009 ; Vol. 124, No. 6. pp. 1358-1365.
@article{048d2be342ca450cb7e72ce4126ae3ab,
title = "Dysregulated microRNAs and their predicted targets associated with endometrioid endometrial adenocarcinoma in Hong Kong women",
abstract = "The objective of this study, a parallel study to global gene expression profiling, was to identify dysregulated microRNAs (miRNAs) associated with endometrioid endometrial adenocarcinoma (EEC), examine their correlation with clinico-pathoiogicai characteristics and identify predicted target genes of the dysregulated miRNAs. Using real-time quantitative reverse transcription-poiy- merase chain reaction (qRT-PCR), profiling of miRNA expression was performed in 30 EECs and 22 normal counterparts in which genome-wide gene expression had been previously profiled and reported. Clustering analysis identified 30 miRNAs which were significantly dysregulated in EEC. The expression of a sub-group of miRNAs was significantly correlated with clinico-pathological characteristics including stage, myometrial invasion, recurrence and lymph node involvement. By searching for predicted miRNA targets that were linked to the dysregulated genes previously identified, 68 genes were predicted as candidate targets of these 30 dysregulated miRNAs. miR-205 was significantly overexpressed in EECs compared with normal controls. After transfection of a miR-205 inhibitor, the expression of miR-205 in endometrial cancer cell line RL95-2 cells decreased whereas its predicted target gene, JPH.4, showed increased protein expression. JPH4 seems to be a real miR-205 target in vitro and in vivo, and a candidate tumor suppressor gene in EEC. Based on this study in EEC, miRNAs predicted to be involved in tumorigenesis and tumor progression have been identified and placed in the context of the transcriptome of EEC. This work provides a framework on which further research into novel diagnosis and treatment of EEC can be focused.",
keywords = "Adenocarcinoma, Endometrial, MicroRNA",
author = "Chung, {Tony K H} and Cheung, {Tak Hong} and Huen, {Ngar Yee} and Wong, {Katherine W Y} and Lo, {Keith W K} and Yim, {So Fan} and Siu, {Nelson S S} and Wong, {Yin Mei} and Tsang, {Po Ting} and Pang, {Man Wah} and Yu, {Mei Yun} and To, {Ka Fei} and Mok, {Samuel C.} and Wang, {Vivian W.} and Chen Li and Cheung, {Albert Y K} and Graeme Doran and Birrer, {Michael J.} and Smith, {David I} and Wong, {Yick Fu}",
year = "2009",
month = "3",
day = "15",
doi = "10.1002/ijc.24071",
language = "English (US)",
volume = "124",
pages = "1358--1365",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "6",

}

TY - JOUR

T1 - Dysregulated microRNAs and their predicted targets associated with endometrioid endometrial adenocarcinoma in Hong Kong women

AU - Chung, Tony K H

AU - Cheung, Tak Hong

AU - Huen, Ngar Yee

AU - Wong, Katherine W Y

AU - Lo, Keith W K

AU - Yim, So Fan

AU - Siu, Nelson S S

AU - Wong, Yin Mei

AU - Tsang, Po Ting

AU - Pang, Man Wah

AU - Yu, Mei Yun

AU - To, Ka Fei

AU - Mok, Samuel C.

AU - Wang, Vivian W.

AU - Li, Chen

AU - Cheung, Albert Y K

AU - Doran, Graeme

AU - Birrer, Michael J.

AU - Smith, David I

AU - Wong, Yick Fu

PY - 2009/3/15

Y1 - 2009/3/15

N2 - The objective of this study, a parallel study to global gene expression profiling, was to identify dysregulated microRNAs (miRNAs) associated with endometrioid endometrial adenocarcinoma (EEC), examine their correlation with clinico-pathoiogicai characteristics and identify predicted target genes of the dysregulated miRNAs. Using real-time quantitative reverse transcription-poiy- merase chain reaction (qRT-PCR), profiling of miRNA expression was performed in 30 EECs and 22 normal counterparts in which genome-wide gene expression had been previously profiled and reported. Clustering analysis identified 30 miRNAs which were significantly dysregulated in EEC. The expression of a sub-group of miRNAs was significantly correlated with clinico-pathological characteristics including stage, myometrial invasion, recurrence and lymph node involvement. By searching for predicted miRNA targets that were linked to the dysregulated genes previously identified, 68 genes were predicted as candidate targets of these 30 dysregulated miRNAs. miR-205 was significantly overexpressed in EECs compared with normal controls. After transfection of a miR-205 inhibitor, the expression of miR-205 in endometrial cancer cell line RL95-2 cells decreased whereas its predicted target gene, JPH.4, showed increased protein expression. JPH4 seems to be a real miR-205 target in vitro and in vivo, and a candidate tumor suppressor gene in EEC. Based on this study in EEC, miRNAs predicted to be involved in tumorigenesis and tumor progression have been identified and placed in the context of the transcriptome of EEC. This work provides a framework on which further research into novel diagnosis and treatment of EEC can be focused.

AB - The objective of this study, a parallel study to global gene expression profiling, was to identify dysregulated microRNAs (miRNAs) associated with endometrioid endometrial adenocarcinoma (EEC), examine their correlation with clinico-pathoiogicai characteristics and identify predicted target genes of the dysregulated miRNAs. Using real-time quantitative reverse transcription-poiy- merase chain reaction (qRT-PCR), profiling of miRNA expression was performed in 30 EECs and 22 normal counterparts in which genome-wide gene expression had been previously profiled and reported. Clustering analysis identified 30 miRNAs which were significantly dysregulated in EEC. The expression of a sub-group of miRNAs was significantly correlated with clinico-pathological characteristics including stage, myometrial invasion, recurrence and lymph node involvement. By searching for predicted miRNA targets that were linked to the dysregulated genes previously identified, 68 genes were predicted as candidate targets of these 30 dysregulated miRNAs. miR-205 was significantly overexpressed in EECs compared with normal controls. After transfection of a miR-205 inhibitor, the expression of miR-205 in endometrial cancer cell line RL95-2 cells decreased whereas its predicted target gene, JPH.4, showed increased protein expression. JPH4 seems to be a real miR-205 target in vitro and in vivo, and a candidate tumor suppressor gene in EEC. Based on this study in EEC, miRNAs predicted to be involved in tumorigenesis and tumor progression have been identified and placed in the context of the transcriptome of EEC. This work provides a framework on which further research into novel diagnosis and treatment of EEC can be focused.

KW - Adenocarcinoma

KW - Endometrial

KW - MicroRNA

UR - http://www.scopus.com/inward/record.url?scp=60549101101&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=60549101101&partnerID=8YFLogxK

U2 - 10.1002/ijc.24071

DO - 10.1002/ijc.24071

M3 - Article

C2 - 19065659

AN - SCOPUS:60549101101

VL - 124

SP - 1358

EP - 1365

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 6

ER -