Dysplastic cells in cytological cervical samples show a high incidence of chromosomal abnormalities

Minghao Song; Adam Ruth; Frank A. Policht; Lukas Bubendorf; Georg Feichter; Benjamin R. Kipp; Kevin C. Halling; Irina A. Sokolova

doi:10.1002/dc.21142

Dysplastic cells in cytological cervical samples show a high incidence of chromosomal abnormalities

Minghao Song, Adam Ruth, Frank A. Policht, Lukas Bubendorf, Georg Feichter, Benjamin R. Kipp, Kevin C. Halling, Irina A. Sokolova

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Chromosomal abnormalities are frequent in most cervical cancers. Amplifications of both the 3q26 (TERC) and 8q24 (MYC) loci have been shown to be prevalent in both high-grade lesions and invasive cervical carcinoma. Most of these studies have looked at either the histological sample or at the entire cytological population of cells. We have developed a Papanicolaou (Pap) destaining method that allows for the accurate analysis of individual cells that were previously identified by cytopathology as dysplastic. The application of fluorescence in situ hybridization (FISH) was then implemented to determine the chromosomal status of the dysplastic cells in the samples and correlate the two events. Chromosomal abnormality is over a thousand times more frequent in dysplastic cells compared with their morphologically normal counterparts.

Original language	English (US)
Pages (from-to)	28-33
Number of pages	6
Journal	Diagnostic cytopathology
Volume	38
Issue number	1
DOIs	https://doi.org/10.1002/dc.21142
State	Published - Jan 2010

Keywords

3q26 (TERC)
8q24 (MYC)
Cervical dysplasia
Chromosomal aberration
FISH
Pap smears

ASJC Scopus subject areas

Pathology and Forensic Medicine
Histology

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10.1002/dc.21142

Cite this

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title = "Dysplastic cells in cytological cervical samples show a high incidence of chromosomal abnormalities",

abstract = "Chromosomal abnormalities are frequent in most cervical cancers. Amplifications of both the 3q26 (TERC) and 8q24 (MYC) loci have been shown to be prevalent in both high-grade lesions and invasive cervical carcinoma. Most of these studies have looked at either the histological sample or at the entire cytological population of cells. We have developed a Papanicolaou (Pap) destaining method that allows for the accurate analysis of individual cells that were previously identified by cytopathology as dysplastic. The application of fluorescence in situ hybridization (FISH) was then implemented to determine the chromosomal status of the dysplastic cells in the samples and correlate the two events. Chromosomal abnormality is over a thousand times more frequent in dysplastic cells compared with their morphologically normal counterparts.",

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AU - Song, Minghao

AU - Ruth, Adam

AU - Policht, Frank A.

AU - Bubendorf, Lukas

AU - Feichter, Georg

AU - Kipp, Benjamin R.

AU - Halling, Kevin C.

AU - Sokolova, Irina A.

PY - 2010/1

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AB - Chromosomal abnormalities are frequent in most cervical cancers. Amplifications of both the 3q26 (TERC) and 8q24 (MYC) loci have been shown to be prevalent in both high-grade lesions and invasive cervical carcinoma. Most of these studies have looked at either the histological sample or at the entire cytological population of cells. We have developed a Papanicolaou (Pap) destaining method that allows for the accurate analysis of individual cells that were previously identified by cytopathology as dysplastic. The application of fluorescence in situ hybridization (FISH) was then implemented to determine the chromosomal status of the dysplastic cells in the samples and correlate the two events. Chromosomal abnormality is over a thousand times more frequent in dysplastic cells compared with their morphologically normal counterparts.

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KW - Chromosomal aberration

KW - FISH

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Dysplastic cells in cytological cervical samples show a high incidence of chromosomal abnormalities

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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