Abstract
Actin reorganization at the immunological synapse is required for the amplification and generation of a functional immune response. Using small interfering RNA, we show here that dynamin 2 (Dyn2), a large GTPase involved in receptor-mediated internalization, did not alter antibody-mediated T cell receptor internalization but considerably affected T cell receptor-stimulated T cell activation by regulating multiple biochemical signaling pathways and the accumulation of F-actin at the immunological synapse. Moreover, Dyn2 interacted directly with the Rho family guanine nucleotide exchange factor Vav1, and this interaction was required for T cell activation. These data identify a functionally important interaction between Dyn2 and Vav1 that regulates actin reorganization and multiple signaling pathways in T lymphocytes.
Original language | English (US) |
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Pages (from-to) | 261-270 |
Number of pages | 10 |
Journal | Nature immunology |
Volume | 6 |
Issue number | 3 |
DOIs | |
State | Published - 2005 |
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology