Dynamin 2 regulates T cell activation by controlling actin polymerization at the immunological synapse

Timothy S. Gomez, Michael J. Hamann, Sean McCarney, Doris N. Savoy, Casey M. Lubking, Michael P. Heldebrant, Christine M. Labno, David J. McKean, Mark A. McNiven, Janis K. Burkhardt, Daniel D. Billadeau

Research output: Contribution to journalArticle

125 Scopus citations

Abstract

Actin reorganization at the immunological synapse is required for the amplification and generation of a functional immune response. Using small interfering RNA, we show here that dynamin 2 (Dyn2), a large GTPase involved in receptor-mediated internalization, did not alter antibody-mediated T cell receptor internalization but considerably affected T cell receptor-stimulated T cell activation by regulating multiple biochemical signaling pathways and the accumulation of F-actin at the immunological synapse. Moreover, Dyn2 interacted directly with the Rho family guanine nucleotide exchange factor Vav1, and this interaction was required for T cell activation. These data identify a functionally important interaction between Dyn2 and Vav1 that regulates actin reorganization and multiple signaling pathways in T lymphocytes.

Original languageEnglish (US)
Pages (from-to)261-270
Number of pages10
JournalNature immunology
Volume6
Issue number3
DOIs
StatePublished - Dec 12 2005

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Gomez, T. S., Hamann, M. J., McCarney, S., Savoy, D. N., Lubking, C. M., Heldebrant, M. P., Labno, C. M., McKean, D. J., McNiven, M. A., Burkhardt, J. K., & Billadeau, D. D. (2005). Dynamin 2 regulates T cell activation by controlling actin polymerization at the immunological synapse. Nature immunology, 6(3), 261-270. https://doi.org/10.1038/ni1168