Dynamin 2 mediates fluid-phase micropinocytosis in epithelial cells

Hong Cao, Jing Chen, Muyiwa Awoniyi, John R Henley, Mark A Mc Niven

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

It is well-known that dynamin 2 (Dyn2) participates in clathrin- and caveolae-mediated endocytosis; however, the role of Dyn2 in coat-independent endocytic processes remains controversial. Here we demonstrate a role for specific spliced variants of Dyn2 in the micropinocytosis of fluid in epithelial cells, independent of coat-mediated endocytic pathways. A general inhibition of Dyn2 was first performed using either microinjection of anti-dynamin antibodies or Dyn2-siRNA treatment. Both of these methods resulted in reduced uptake of transferrin, a marker for clathrin-mediated endocytosis, and, under unstimulated conditions, reduced the uptake of the fluid-phase markers dextran and horseradish peroxidase (HRP). By contrast, cells treated similarly but stimulated with serum or EGF internalized substantial amounts of dextran or HRP, indicating that Dyn2 is not required for stimulated fluid uptake via macropinocytosis. We next tested whether a specific spliced variant might selectively affect fluid-phase endocytosis. Mutation of specific Dyn2 spliced variants resulted in a differential attenuation of transferrin and dextran internalization. Furthermore, the reduction in fluid uptake in Dyn2-siRNA-treated cells was only rescued upon re-expression of select spliced variants. These findings suggest that Dyn2 function is required for the coat-independent internalization of fluid through endocytic pathways distinct from macropinocytosis and, in addition, implicate different Dyn2 spliced variants in specific endocytic functions.

Original languageEnglish (US)
Pages (from-to)4167-4177
Number of pages11
JournalJournal of Cell Science
Volume120
Issue number23
DOIs
StatePublished - Dec 1 2007

Fingerprint

Dynamin II
Epithelial Cells
Endocytosis
Dextrans
Clathrin
Horseradish Peroxidase
Transferrin
Small Interfering RNA
Dynamins
Glycocalyx
Caveolae
Microinjections
Epidermal Growth Factor
Anti-Idiotypic Antibodies

Keywords

  • Clathrin-independent
  • Dynamin 2
  • Fluid-phase endocytosis
  • Macropinocytosis
  • Micropinocytosis
  • Spliced variants

ASJC Scopus subject areas

  • Cell Biology

Cite this

Dynamin 2 mediates fluid-phase micropinocytosis in epithelial cells. / Cao, Hong; Chen, Jing; Awoniyi, Muyiwa; Henley, John R; Mc Niven, Mark A.

In: Journal of Cell Science, Vol. 120, No. 23, 01.12.2007, p. 4167-4177.

Research output: Contribution to journalArticle

Cao, Hong ; Chen, Jing ; Awoniyi, Muyiwa ; Henley, John R ; Mc Niven, Mark A. / Dynamin 2 mediates fluid-phase micropinocytosis in epithelial cells. In: Journal of Cell Science. 2007 ; Vol. 120, No. 23. pp. 4167-4177.
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