Dynamics of palmitic acid complexed with rat intestinal fatty acid binding protein

Lingyang Zhu, Elizabeth Kurian, Franklyn G. Prendergast, Marvin D. Kemple

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Abstract

Dynamics of palmitic acid (PA), isotopically enriched with 13C at the second, seventh, or terminal methyl position, were investigated by 13C NMR. Relaxation measurements were made on PA bound to recombinant rat intestinal fatty acid binding protein (I-FABP) at pH 5.5 and 23 °(Degree Sign)C, and, for comparison, on PA incorporated into 1-palmitoyl-2-hydroxy-sn-glycero-3- phosphocholine (MPPC) micelles, and dissolved in methanol. The 13C relaxation data, T1, and steady-state nuclear Overhauser effect (NOE) obtained at two different magnetic fields were interpreted using the model- free approach [Lipari, G., and Szabo, A. (1982) J. Am. Chem. Soc. 104, 4546- 4559]. The overall rotational correlation time of the fatty acid·protein complex was 2.5 ± 0.4 ns, which is substantially less than the value expected for the protein itself (>6 ns). Order parameters (S2), which are a measure of the amplitude of the internal motion of individual C-H vectors with respect to the PA molecule, while largest for C-2 and smallest for the methyl carbon, were relatively small (<0.4) in the protein complex. S2 values for given C-H vectors also were smaller for PA in the MPPC micelles and in methanol than in the protein complex. Correlation times reflective of the time scale of the internal motion of the C-H vectors were in all cases <60 ps. These results support the view that the fatty acid is not rigidly anchored within the I-FABP binding pocket, but rather has considerable freedom to move within the pocket.

Original languageEnglish (US)
Pages (from-to)1554-1561
Number of pages8
JournalBiochemistry
Volume38
Issue number5
DOIs
StatePublished - Feb 2 1999

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ASJC Scopus subject areas

  • Biochemistry

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Zhu, L., Kurian, E., Prendergast, F. G., & Kemple, M. D. (1999). Dynamics of palmitic acid complexed with rat intestinal fatty acid binding protein. Biochemistry, 38(5), 1554-1561. https://doi.org/10.1021/bi982087v