Dynamics of MRI lesion development in an animal model of viral-induced acute progressive CNS demyelination

Istvan Pirko, Jeff Gamez, Aaron J. Johnson, Slobodan I Macura, Moses Rodriguez

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Theiler's murine encephalitis virus (TMEV) infection in mice is an established model of CNS demyelinating diseases. The aim of the study was to determine the chronological pattern of lesion development in this model of monophasic fulminant demyelinating disease. We followed six highly susceptible interferon-gamma receptor knockout mice with serial in vivo brain magnetic resonance imaging (MRI) studies to determine changes in overall T2 lesion load and gadolinium enhancement. Altogether, 163 individual lesions were followed over 52 days. The number of lesions increased linearly with time. Four chronological patterns of lesion development were seen: (a) expanding lesions (48.5% of all lesions, 54.05% volume contribution); (b) expanding-retracting lesions (20.85% of all lesions, 15.03% volume contribution); (c) fluctuating lesions (16.6% of all lesions, 28.8% volume contribution); (d) stable lesions (14.05% of all lesions, 2.12% volume contribution). Gadolinium enhancement was not seen in the evolution of every lesion. Enhancement was both time- and lesion type-dependent. Early in the disease course (<43 days after infection), enhancement was almost always seen, later on (>43 days after infection) it was only seen in 8% of new lesions. All of fluctuating, 85.3% of expanding, 83.5% of expanding-retracting, and 56.5% of stable lesions were associated with gadolinium enhancement. We conclude that the MRI features of TMEV-induced demyelination in this model showed four unique chronological patterns, and inconsistent gadolinium enhancement. These novel findings may provide new insights into the pathogenesis of acute fulminant multiple sclerosis (MS).

Original languageEnglish (US)
Pages (from-to)576-582
Number of pages7
JournalNeuroImage
Volume21
Issue number2
DOIs
StatePublished - Feb 2004

Fingerprint

Gadolinium
Demyelinating Diseases
Animal Models
Encephalitis Viruses
Magnetic Resonance Imaging
Central Nervous System Diseases
Virus Diseases
Knockout Mice
Multiple Sclerosis
Brain
Infection

Keywords

  • CNS demyelination
  • Lesion development
  • MRI

ASJC Scopus subject areas

  • Cognitive Neuroscience
  • Neurology

Cite this

Dynamics of MRI lesion development in an animal model of viral-induced acute progressive CNS demyelination. / Pirko, Istvan; Gamez, Jeff; Johnson, Aaron J.; Macura, Slobodan I; Rodriguez, Moses.

In: NeuroImage, Vol. 21, No. 2, 02.2004, p. 576-582.

Research output: Contribution to journalArticle

@article{6430aede9ae14143afe55f5764782d9a,
title = "Dynamics of MRI lesion development in an animal model of viral-induced acute progressive CNS demyelination",
abstract = "Theiler's murine encephalitis virus (TMEV) infection in mice is an established model of CNS demyelinating diseases. The aim of the study was to determine the chronological pattern of lesion development in this model of monophasic fulminant demyelinating disease. We followed six highly susceptible interferon-gamma receptor knockout mice with serial in vivo brain magnetic resonance imaging (MRI) studies to determine changes in overall T2 lesion load and gadolinium enhancement. Altogether, 163 individual lesions were followed over 52 days. The number of lesions increased linearly with time. Four chronological patterns of lesion development were seen: (a) expanding lesions (48.5{\%} of all lesions, 54.05{\%} volume contribution); (b) expanding-retracting lesions (20.85{\%} of all lesions, 15.03{\%} volume contribution); (c) fluctuating lesions (16.6{\%} of all lesions, 28.8{\%} volume contribution); (d) stable lesions (14.05{\%} of all lesions, 2.12{\%} volume contribution). Gadolinium enhancement was not seen in the evolution of every lesion. Enhancement was both time- and lesion type-dependent. Early in the disease course (<43 days after infection), enhancement was almost always seen, later on (>43 days after infection) it was only seen in 8{\%} of new lesions. All of fluctuating, 85.3{\%} of expanding, 83.5{\%} of expanding-retracting, and 56.5{\%} of stable lesions were associated with gadolinium enhancement. We conclude that the MRI features of TMEV-induced demyelination in this model showed four unique chronological patterns, and inconsistent gadolinium enhancement. These novel findings may provide new insights into the pathogenesis of acute fulminant multiple sclerosis (MS).",
keywords = "CNS demyelination, Lesion development, MRI",
author = "Istvan Pirko and Jeff Gamez and Johnson, {Aaron J.} and Macura, {Slobodan I} and Moses Rodriguez",
year = "2004",
month = "2",
doi = "10.1016/j.neuroimage.2003.09.037",
language = "English (US)",
volume = "21",
pages = "576--582",
journal = "NeuroImage",
issn = "1053-8119",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Dynamics of MRI lesion development in an animal model of viral-induced acute progressive CNS demyelination

AU - Pirko, Istvan

AU - Gamez, Jeff

AU - Johnson, Aaron J.

AU - Macura, Slobodan I

AU - Rodriguez, Moses

PY - 2004/2

Y1 - 2004/2

N2 - Theiler's murine encephalitis virus (TMEV) infection in mice is an established model of CNS demyelinating diseases. The aim of the study was to determine the chronological pattern of lesion development in this model of monophasic fulminant demyelinating disease. We followed six highly susceptible interferon-gamma receptor knockout mice with serial in vivo brain magnetic resonance imaging (MRI) studies to determine changes in overall T2 lesion load and gadolinium enhancement. Altogether, 163 individual lesions were followed over 52 days. The number of lesions increased linearly with time. Four chronological patterns of lesion development were seen: (a) expanding lesions (48.5% of all lesions, 54.05% volume contribution); (b) expanding-retracting lesions (20.85% of all lesions, 15.03% volume contribution); (c) fluctuating lesions (16.6% of all lesions, 28.8% volume contribution); (d) stable lesions (14.05% of all lesions, 2.12% volume contribution). Gadolinium enhancement was not seen in the evolution of every lesion. Enhancement was both time- and lesion type-dependent. Early in the disease course (<43 days after infection), enhancement was almost always seen, later on (>43 days after infection) it was only seen in 8% of new lesions. All of fluctuating, 85.3% of expanding, 83.5% of expanding-retracting, and 56.5% of stable lesions were associated with gadolinium enhancement. We conclude that the MRI features of TMEV-induced demyelination in this model showed four unique chronological patterns, and inconsistent gadolinium enhancement. These novel findings may provide new insights into the pathogenesis of acute fulminant multiple sclerosis (MS).

AB - Theiler's murine encephalitis virus (TMEV) infection in mice is an established model of CNS demyelinating diseases. The aim of the study was to determine the chronological pattern of lesion development in this model of monophasic fulminant demyelinating disease. We followed six highly susceptible interferon-gamma receptor knockout mice with serial in vivo brain magnetic resonance imaging (MRI) studies to determine changes in overall T2 lesion load and gadolinium enhancement. Altogether, 163 individual lesions were followed over 52 days. The number of lesions increased linearly with time. Four chronological patterns of lesion development were seen: (a) expanding lesions (48.5% of all lesions, 54.05% volume contribution); (b) expanding-retracting lesions (20.85% of all lesions, 15.03% volume contribution); (c) fluctuating lesions (16.6% of all lesions, 28.8% volume contribution); (d) stable lesions (14.05% of all lesions, 2.12% volume contribution). Gadolinium enhancement was not seen in the evolution of every lesion. Enhancement was both time- and lesion type-dependent. Early in the disease course (<43 days after infection), enhancement was almost always seen, later on (>43 days after infection) it was only seen in 8% of new lesions. All of fluctuating, 85.3% of expanding, 83.5% of expanding-retracting, and 56.5% of stable lesions were associated with gadolinium enhancement. We conclude that the MRI features of TMEV-induced demyelination in this model showed four unique chronological patterns, and inconsistent gadolinium enhancement. These novel findings may provide new insights into the pathogenesis of acute fulminant multiple sclerosis (MS).

KW - CNS demyelination

KW - Lesion development

KW - MRI

UR - http://www.scopus.com/inward/record.url?scp=1242271385&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1242271385&partnerID=8YFLogxK

U2 - 10.1016/j.neuroimage.2003.09.037

DO - 10.1016/j.neuroimage.2003.09.037

M3 - Article

C2 - 14980559

AN - SCOPUS:1242271385

VL - 21

SP - 576

EP - 582

JO - NeuroImage

JF - NeuroImage

SN - 1053-8119

IS - 2

ER -