Dynamics of chromosome spreading

Jack L. Spurbeck, Alan R. Zinsmeister, Kevin J. Meyer, Syed M. Jalal

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

Consistency of optimum chromosome spreading during harvest of cytogenetic specimens remains a major concern. We have tested the idea that a precise control of the drying rate (the time with which metaphase cells dry), as fixed cell suspension is placed on a slide or an in situ culture in last fixation, may be the answer. Amniocyte and lymphocyte cultures were allowed to dry at defined combinations of relative humidity (RH) and temperature (T) in a modified Thermotron environmental control unit. We were able to demonstrate, based on 2,250 amniocytes and 1,650 lymphocytes, that the metaphase area after drying was a function of RH and T for both in situ and non-in situ culture systems. As the RH and T increase, the metaphase area increases until a threshold is reached. Also, as RH increases, the slide drying time increases. Data obtained using a response surface regression, proportional hazards regression analysis and slide drying time studies are consistent with our model of chromosome spreading. Optimum metaphase areas can be achieved at various combinations of RH and T. We propose that the use of an environmental control unit is a practical way of achieving optimum chromosome spreading routinely and in a highly consistent manner.

Original languageEnglish (US)
Pages (from-to)387-393
Number of pages7
JournalAmerican journal of medical genetics
Volume61
Issue number4
DOIs
StatePublished - Feb 2 1996

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Keywords

  • chromosome harvest chamber
  • chromosome spreading
  • environmental control
  • optimum cytogenetic harvesting

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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