Dynamic and tissue-specific expression of eIF4E during zebrafish embryogenesis

Scott C. Fahrenkrug, Mark O. Dahlquist, Karl J Clark, Perry B. Hackett

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The regulation of protein synthesis is critical to diverse cellular processes and plays a pivotal role in regulating gene expression during embryogenesis. The cap-binding protein eIF4E is a translational factor whose activity appears to be both ubiquitous and central to the regulation of protein synthesis in all cell-types. As a cell-cycle regulator, mesoderm inducer and proto-oncogene, the amount and activity of the translational factor eIF4E must be under strict control, but the range of its expression and its concentration as a function of position and time in the developing embryo are unknown. Consequently, we have initiated studies to elucidate the expression of the eIF4E gene and its role in the regulating embryonic development. We have cloned a zebrafish gene encoding eIF4E, zeIF4E, and measured its developmental expression. Unexpectedly, we found that the zeIF4E gene produces two alternatively spliced transcripts that potentially encode different forms of the initiation factor. Molecular analyses and in situ hybridization reveal a potential role for eIF4E in regulating protein synthesis during vertebrate oogenesis, gastrulation, and erythropoiesis. The dynamic and asymmetric expression of eIF4E during zebrafish embryogenesis reveals that this ostensibly general translation factor may act as a tissue-specific translational enhancer.

Original languageEnglish (US)
Pages (from-to)191-201
Number of pages11
JournalDifferentiation
Volume65
Issue number4
StatePublished - 1999
Externally publishedYes

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Zebrafish
Embryonic Development
RNA Cap-Binding Proteins
Gene Expression
Oogenesis
Peptide Initiation Factors
Gastrulation
Proteins
Proto-Oncogenes
Erythropoiesis
Mesoderm
Genes
In Situ Hybridization
Vertebrates
Cell Cycle
Embryonic Structures

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Developmental Biology
  • Cell Biology

Cite this

Fahrenkrug, S. C., Dahlquist, M. O., Clark, K. J., & Hackett, P. B. (1999). Dynamic and tissue-specific expression of eIF4E during zebrafish embryogenesis. Differentiation, 65(4), 191-201.

Dynamic and tissue-specific expression of eIF4E during zebrafish embryogenesis. / Fahrenkrug, Scott C.; Dahlquist, Mark O.; Clark, Karl J; Hackett, Perry B.

In: Differentiation, Vol. 65, No. 4, 1999, p. 191-201.

Research output: Contribution to journalArticle

Fahrenkrug, SC, Dahlquist, MO, Clark, KJ & Hackett, PB 1999, 'Dynamic and tissue-specific expression of eIF4E during zebrafish embryogenesis', Differentiation, vol. 65, no. 4, pp. 191-201.
Fahrenkrug, Scott C. ; Dahlquist, Mark O. ; Clark, Karl J ; Hackett, Perry B. / Dynamic and tissue-specific expression of eIF4E during zebrafish embryogenesis. In: Differentiation. 1999 ; Vol. 65, No. 4. pp. 191-201.
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