DYF-4 regulates patched-related/DAF-6- mediated sensory compartment formation in C. elegans

Hui Hong, Huicheng Chen, Yuxia Zhang, Zhimao Wu, Yingying Zhang, Yingyi Zhang, Zeng Hu, Jian V. Zhang, Kun Ling, Jinghua Hu, Qing Wei

Research output: Contribution to journalArticlepeer-review


Coordination of neurite extension with surrounding glia development is critical for neuronal function, but the underlying molecular mechanisms remain poorly understood. Through a genome-wide mutagenesis screen in C. elegans, we identified dyf-4 and daf-6 as two mutants sharing similar defects in dendrite extension. DAF-6 encodes a glia-specific patched-related membrane protein that plays vital roles in glial morphogenesis. We cloned dyf-4 and found that DYF-4 encodes a glia-secreted protein. Further investigations revealed that DYF-4 interacts with DAF-6 and functions in a same pathway as DAF-6 to regulate sensory compartment formation. Furthermore, we demonstrated that reported glial suppressors of daf-6 could also restore dendrite elongation and ciliogenesis in both dyf-4 and daf-6 mutants. Collectively, our data reveal that DYF-4 is a regulator for DAF-6 which promotes the proper formation of the glial channel and indirectly affects neurite extension and ciliogenesis.

Original languageEnglish (US)
Article numbere1009618
JournalPLoS genetics
Issue number6
StatePublished - Jun 11 2021

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research


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