Duodenoesophageal reflux induces apoptosis in rat esophageal epithelium

Natsuya Katada, Ronald A. Hinder, Thomas Christopher Smyrk, Yoshiki Hiki, Akiri Kakita

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The esophageal epithelial damage caused by duodenoesophageal reflux was examined by assessing histology, free radicals, and apoptosis in rats with an end-to-side esophagoduodenostomy (reflux group) or sham operation. Rats were sacrificed at 1, 6, or 12 weeks after surgery. Reflux-associated hyperplasia was noted in the reflux group at 6 and 12 weeks. The reflux group manifested glutathione (GSH) overproduction at one and six weeks. Apoptotic cells were detected by the terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end-labeling method. Significantly increased numbers of apoptotic cells were seen on the epithelial surface in the reflux group at 6 and 12 weeks. Duodenoesophageal reflux causes esophageal injury with hyperplasia. Early GSH overproduction indicated that reflux esophagitis may be partly mediated by free radicals. Increased apoptosis may counteract increased proliferation and may be a protective mechanism against increased genotoxic events.

Original languageEnglish (US)
Pages (from-to)301-310
Number of pages10
JournalDigestive Diseases and Sciences
Volume44
Issue number2
StatePublished - 1999

Fingerprint

Free Radicals
Hyperplasia
Epithelium
Apoptosis
Peptic Esophagitis
DNA Nucleotidylexotransferase
Biotin
Gastroesophageal Reflux
Glutathione
Histology
Cell Count
Wounds and Injuries

Keywords

  • Apoptosis
  • Duodenoesophageal reflux
  • Free radicals
  • Hyperplasia

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Katada, N., Hinder, R. A., Smyrk, T. C., Hiki, Y., & Kakita, A. (1999). Duodenoesophageal reflux induces apoptosis in rat esophageal epithelium. Digestive Diseases and Sciences, 44(2), 301-310.

Duodenoesophageal reflux induces apoptosis in rat esophageal epithelium. / Katada, Natsuya; Hinder, Ronald A.; Smyrk, Thomas Christopher; Hiki, Yoshiki; Kakita, Akiri.

In: Digestive Diseases and Sciences, Vol. 44, No. 2, 1999, p. 301-310.

Research output: Contribution to journalArticle

Katada, N, Hinder, RA, Smyrk, TC, Hiki, Y & Kakita, A 1999, 'Duodenoesophageal reflux induces apoptosis in rat esophageal epithelium', Digestive Diseases and Sciences, vol. 44, no. 2, pp. 301-310.
Katada N, Hinder RA, Smyrk TC, Hiki Y, Kakita A. Duodenoesophageal reflux induces apoptosis in rat esophageal epithelium. Digestive Diseases and Sciences. 1999;44(2):301-310.
Katada, Natsuya ; Hinder, Ronald A. ; Smyrk, Thomas Christopher ; Hiki, Yoshiki ; Kakita, Akiri. / Duodenoesophageal reflux induces apoptosis in rat esophageal epithelium. In: Digestive Diseases and Sciences. 1999 ; Vol. 44, No. 2. pp. 301-310.
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