Dual signaling and effector pathways mediate human eosinophil activation by platelet-activating factor

Masahiko Kato, Hirohito Kita, Atsushi Tachibana, Yasuhide Hayashi, Yoshiaki Tsuchida, Hirokazu Kimura

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Platelet-activating factor (PAF) induces various cellular functions in eosinophils including chemotaxis, adhesion, superoxide anion (O2-) production, and degranulation. While PAF shares many biological effects with other chemotactic factors such as N-formyl-methionyl-leucyl-phenylalanine, complement fragments, and lipid mediators, PAF is unique in that its action is relatively resistant to pertussis toxin (PTX), and in activating eosinophils more strongly than neutrophils. In this review we consider how PAF might activate human eosinophils in preference to neutrophils, and discuss possible mechanisms of PAF-induced activation of human eosinophils via two distinct signaling and effector pathways. Recently we analyzed O2- production by eosinophils using a sensitive, real-time chemiluminescence method. Our results showed that in human eosinophils PAF activates two distinct signaling and effector pathways coupled to the PAF receptor: one linked to PTX-sensitive G protein(s) and another to PTX-resistant G protein(s), phosphatidylinositol 3-kinase, and cellular adhesion. This activation of two different G proteins by the eosinophil PAF receptor may explain the strong and diverse biological responses of human eosinophils to PAF.

Original languageEnglish (US)
Pages (from-to)37-43
Number of pages7
JournalInternational archives of allergy and immunology
Volume134
Issue numberSUPPL. 1
DOIs
StatePublished - Jun 15 2004

Keywords

  • Eosinophils
  • Neutrophils
  • Platelet-activating factor
  • Signal transduction

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Dual signaling and effector pathways mediate human eosinophil activation by platelet-activating factor'. Together they form a unique fingerprint.

  • Cite this