TY - JOUR
T1 - Dual antimicrobial-loaded biodegradable nanoemulsions for synergistic treatment of wound biofilms
AU - Nabawy, Ahmed
AU - Makabenta, Jessa Marie
AU - Schmidt-Malan, Suzannah
AU - Park, Jungmi
AU - Li, Cheng Hsuan
AU - Huang, Rui
AU - Fedeli, Stefano
AU - Chattopadhyay, Aritra Nath
AU - Patel, Robin
AU - Rotello, Vincent M.
N1 - Funding Information:
This research was supported by the National Institutes of Health under R01 AI134770. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Clinical samples obtained from the Cooley Dickinson Hospital Microbiology Laboratory (Northampton, MA) were kindly provided by Dr. Margaret Riley. Bioluminescent MRSA USA300 NRS384 strain, SAP-231, was generously provided by Dr. Roger Plaut. The microscopy data was gathered in the Light Microscopy Facility and Nikon Center of Excellence at the Institute for Applied Life Sciences.
Funding Information:
This research was supported by the National Institutes of Health under R01 AI134770 . The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health . Clinical samples obtained from the Cooley Dickinson Hospital Microbiology Laboratory (Northampton, MA) were kindly provided by Dr. Margaret Riley. Bioluminescent MRSA USA300 NRS384 strain, SAP-231, was generously provided by Dr. Roger Plaut. The microscopy data was gathered in the Light Microscopy Facility and Nikon Center of Excellence at the Institute for Applied Life Sciences .
Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/7
Y1 - 2022/7
N2 - Wound biofilm infections caused by multidrug-resistant (MDR) bacteria constitute a major threat to public health; acquired resistance combined with resistance associated with the biofilm phenotype makes combatting these infections challenging. Biodegradable polymeric nanoemulsions that encapsulate two hydrophobic antimicrobial agents (eugenol and triclosan) (TE-BNEs) as a strategy to combat chronic wound infections are reported here. The cationic nanoemulsions efficiently penetrate and accumulate in biofilms, synergistically eradicating MDR bacterial biofilms, including persister cells. Notably, the nanoemulsion platform displays excellent biocompatibility and delays emergence of resistance to triclosan. The TE-BNEs are active in an in vivo murine model of mature MDR wound biofilm infections, with 99% bacterial elimination. The efficacy of this system coupled with prevention of emergence of bacterial resistance highlight the potential of this combination platform to treat MDR wound biofilm infections.
AB - Wound biofilm infections caused by multidrug-resistant (MDR) bacteria constitute a major threat to public health; acquired resistance combined with resistance associated with the biofilm phenotype makes combatting these infections challenging. Biodegradable polymeric nanoemulsions that encapsulate two hydrophobic antimicrobial agents (eugenol and triclosan) (TE-BNEs) as a strategy to combat chronic wound infections are reported here. The cationic nanoemulsions efficiently penetrate and accumulate in biofilms, synergistically eradicating MDR bacterial biofilms, including persister cells. Notably, the nanoemulsion platform displays excellent biocompatibility and delays emergence of resistance to triclosan. The TE-BNEs are active in an in vivo murine model of mature MDR wound biofilm infections, with 99% bacterial elimination. The efficacy of this system coupled with prevention of emergence of bacterial resistance highlight the potential of this combination platform to treat MDR wound biofilm infections.
KW - Antimicrobials
KW - In vivo
KW - Multidrug-resistant bacteria
KW - Nanoemulsions
KW - Wound biofilms
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U2 - 10.1016/j.jconrel.2022.05.013
DO - 10.1016/j.jconrel.2022.05.013
M3 - Article
C2 - 35550914
AN - SCOPUS:85130554069
SN - 0168-3659
VL - 347
SP - 379
EP - 388
JO - Journal of Controlled Release
JF - Journal of Controlled Release
ER -