Drosophila nerve cord culture: A tool for studying neural development

Y. E. Wang, R. Chandler, P. Lau, A. J. Bieber

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

The culture of explanted neural tissues has been a useful tool for the study of cellular and molecular neurobiology in vertebrates. We have developed a technique for the culture of explanted ventral nerve cords from Drosophila embryos. We have examined the morphology and dynamic behaviour of the growth cones that extend from these nerve cords, and the effect of calcium deprivation on the bundling of axons that are regenerated from the explanted tissue. This technique offers a unique opportunity to combine in vitro techniques for neuronal cell culture with the powerful techniques of genetic analysis that are available with Drosophila.

Original languageEnglish (US)
Pages (from-to)21-26
Number of pages6
JournalJournal of Neuroscience Methods
Volume85
Issue number1
DOIs
StatePublished - Nov 1 1998

Keywords

  • Axon bundling
  • Calcium
  • Drosophila
  • Growth cone
  • Microtubule
  • Neurite outgrowth
  • Neuronal tissue culture

ASJC Scopus subject areas

  • Neuroscience(all)

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