Droplet digital PCR for quantitation of extracellular RNA

Irene K. Yan, Rishabh Lohray, Tushar C Patel

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Cell-to-cell communication involves the release of biological molecules into the extracellular space and their uptake by recipient cells. These molecules include RNA that, can modulate cellular signaling and biological processes. To study extracellular RNA, highly sensitive and precise methods, for their detection are needed. Digital polymerase chain reaction (dPCR) can be a useful method for detecting and analyzing extracellular RNA. The sensitivity of digital PCR can exceed that of quantitative PCR for low abundance targets such as extracellular RNA.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages155-162
Number of pages8
Volume1740
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1740
ISSN (Print)1064-3745

Keywords

  • Digital PCR
  • Extracellular RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Yan, I. K., Lohray, R., & Patel, T. C. (2018). Droplet digital PCR for quantitation of extracellular RNA. In Methods in Molecular Biology (Vol. 1740, pp. 155-162). (Methods in Molecular Biology; Vol. 1740). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7652-2_12