Droplet digital PCR for quantitation of extracellular RNA

Irene K. Yan, Rishabh Lohray, Tushar C Patel

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Cell-to-cell communication involves the release of biological molecules into the extracellular space and their uptake by recipient cells. These molecules include RNA that, can modulate cellular signaling and biological processes. To study extracellular RNA, highly sensitive and precise methods, for their detection are needed. Digital polymerase chain reaction (dPCR) can be a useful method for detecting and analyzing extracellular RNA. The sensitivity of digital PCR can exceed that of quantitative PCR for low abundance targets such as extracellular RNA.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages155-162
Number of pages8
Volume1740
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1740
ISSN (Print)1064-3745

Fingerprint

RNA
Polymerase Chain Reaction
Biological Phenomena
Extracellular Space
Cell Communication

Keywords

  • Digital PCR
  • Extracellular RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Yan, I. K., Lohray, R., & Patel, T. C. (2018). Droplet digital PCR for quantitation of extracellular RNA. In Methods in Molecular Biology (Vol. 1740, pp. 155-162). (Methods in Molecular Biology; Vol. 1740). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7652-2_12

Droplet digital PCR for quantitation of extracellular RNA. / Yan, Irene K.; Lohray, Rishabh; Patel, Tushar C.

Methods in Molecular Biology. Vol. 1740 Humana Press Inc., 2018. p. 155-162 (Methods in Molecular Biology; Vol. 1740).

Research output: Chapter in Book/Report/Conference proceedingChapter

Yan, IK, Lohray, R & Patel, TC 2018, Droplet digital PCR for quantitation of extracellular RNA. in Methods in Molecular Biology. vol. 1740, Methods in Molecular Biology, vol. 1740, Humana Press Inc., pp. 155-162. https://doi.org/10.1007/978-1-4939-7652-2_12
Yan IK, Lohray R, Patel TC. Droplet digital PCR for quantitation of extracellular RNA. In Methods in Molecular Biology. Vol. 1740. Humana Press Inc. 2018. p. 155-162. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7652-2_12
Yan, Irene K. ; Lohray, Rishabh ; Patel, Tushar C. / Droplet digital PCR for quantitation of extracellular RNA. Methods in Molecular Biology. Vol. 1740 Humana Press Inc., 2018. pp. 155-162 (Methods in Molecular Biology).
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