Dopamine release modifies intracellular calcium levels in tyrosine hydroxylase-transfected C6 cells

Hugo Guerrero Cazares, María del Pilar Alatorre-Carranza, Vidal Delgado-Rizo, Judith Marcela Duenas-Jimenez, Maria Luisa Mendoza-Magana, Alberto Morales-Villagran, Mario A. Ramirez-Herrera, Agustin Guerrero-Hernández, Jose Segovia, Sergio H. Duenas-Jimenez

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Glioma cell line C6, transfected with tyrosine hydroxylase (TH) cDNA under the control of the glial fibrillary acid protein promoter (C6-THA cells), elicited a reduction in the apomorphine-induced turning behavior when they are implanted in Parkinson's disease models. Nevertheless, dopamine (Da) release has not been explicitly demonstrated nor has a possible mechanism of release been implicated. In this study, the in vitro Da release by C6 and C6-THA cells after chemical stimulation with KCl or glutamate was quantified using HPLC. Modifications in intracellular calcium levels in response to KCl stimulation and participation of Da receptor-mediated feedback in calcium regulation were also studied using FLUO 3 as a calcium concentration indicator. C6-THA cells release dopamine in basal conditions, and increase its release after KCl or glutamic acid stimulation. In a fraction of C6 and C6-THA cells, a transient intracellular calcium increase was observed after KCl stimulation, but C6-THA cells demonstrated a faster rate of calcium removal. C6 cells express mRNA from all five subtypes of Da receptors as demonstrated by real time PCR. D1 receptors were most abundant in C6 cells and its expression was further increased in C6-THA cells. Blocking D1-like receptors in C6-THA cells with the specific antagonist drug SCH-23390 induced a decrease in intracellular calcium removal rate, resembling non-manipulated C6 cells' calcium clearance. Da release by C6-THA cells could be related to calcium dependent mechanisms. Furthermore, production of Da by C6-THA cells seems to upregulate the expression of D1 receptors' mRNA.

Original languageEnglish (US)
Pages (from-to)113-118
Number of pages6
JournalBrain Research Bulletin
Volume74
Issue number1-3
DOIs
StatePublished - Sep 14 2007
Externally publishedYes

Fingerprint

Tyrosine 3-Monooxygenase
Tacrine
Dopamine
Calcium
Dopamine Receptors
Glutamic Acid
Chemical Stimulation
Messenger RNA
Apomorphine
Glial Fibrillary Acidic Protein
Glioma
Parkinson Disease
Real-Time Polymerase Chain Reaction
Up-Regulation
Complementary DNA
High Pressure Liquid Chromatography

Keywords

  • Dopamine receptors
  • Fluo-3
  • Glial cells
  • L-745,870
  • Neurotransmitter release
  • SCH-23390

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Guerrero Cazares, H., Alatorre-Carranza, M. D. P., Delgado-Rizo, V., Duenas-Jimenez, J. M., Mendoza-Magana, M. L., Morales-Villagran, A., ... Duenas-Jimenez, S. H. (2007). Dopamine release modifies intracellular calcium levels in tyrosine hydroxylase-transfected C6 cells. Brain Research Bulletin, 74(1-3), 113-118. https://doi.org/10.1016/j.brainresbull.2007.05.008

Dopamine release modifies intracellular calcium levels in tyrosine hydroxylase-transfected C6 cells. / Guerrero Cazares, Hugo; Alatorre-Carranza, María del Pilar; Delgado-Rizo, Vidal; Duenas-Jimenez, Judith Marcela; Mendoza-Magana, Maria Luisa; Morales-Villagran, Alberto; Ramirez-Herrera, Mario A.; Guerrero-Hernández, Agustin; Segovia, Jose; Duenas-Jimenez, Sergio H.

In: Brain Research Bulletin, Vol. 74, No. 1-3, 14.09.2007, p. 113-118.

Research output: Contribution to journalArticle

Guerrero Cazares, H, Alatorre-Carranza, MDP, Delgado-Rizo, V, Duenas-Jimenez, JM, Mendoza-Magana, ML, Morales-Villagran, A, Ramirez-Herrera, MA, Guerrero-Hernández, A, Segovia, J & Duenas-Jimenez, SH 2007, 'Dopamine release modifies intracellular calcium levels in tyrosine hydroxylase-transfected C6 cells', Brain Research Bulletin, vol. 74, no. 1-3, pp. 113-118. https://doi.org/10.1016/j.brainresbull.2007.05.008
Guerrero Cazares H, Alatorre-Carranza MDP, Delgado-Rizo V, Duenas-Jimenez JM, Mendoza-Magana ML, Morales-Villagran A et al. Dopamine release modifies intracellular calcium levels in tyrosine hydroxylase-transfected C6 cells. Brain Research Bulletin. 2007 Sep 14;74(1-3):113-118. https://doi.org/10.1016/j.brainresbull.2007.05.008
Guerrero Cazares, Hugo ; Alatorre-Carranza, María del Pilar ; Delgado-Rizo, Vidal ; Duenas-Jimenez, Judith Marcela ; Mendoza-Magana, Maria Luisa ; Morales-Villagran, Alberto ; Ramirez-Herrera, Mario A. ; Guerrero-Hernández, Agustin ; Segovia, Jose ; Duenas-Jimenez, Sergio H. / Dopamine release modifies intracellular calcium levels in tyrosine hydroxylase-transfected C6 cells. In: Brain Research Bulletin. 2007 ; Vol. 74, No. 1-3. pp. 113-118.
@article{0b029ec11cc8483ca8ce7e1ad252928a,
title = "Dopamine release modifies intracellular calcium levels in tyrosine hydroxylase-transfected C6 cells",
abstract = "Glioma cell line C6, transfected with tyrosine hydroxylase (TH) cDNA under the control of the glial fibrillary acid protein promoter (C6-THA cells), elicited a reduction in the apomorphine-induced turning behavior when they are implanted in Parkinson's disease models. Nevertheless, dopamine (Da) release has not been explicitly demonstrated nor has a possible mechanism of release been implicated. In this study, the in vitro Da release by C6 and C6-THA cells after chemical stimulation with KCl or glutamate was quantified using HPLC. Modifications in intracellular calcium levels in response to KCl stimulation and participation of Da receptor-mediated feedback in calcium regulation were also studied using FLUO 3 as a calcium concentration indicator. C6-THA cells release dopamine in basal conditions, and increase its release after KCl or glutamic acid stimulation. In a fraction of C6 and C6-THA cells, a transient intracellular calcium increase was observed after KCl stimulation, but C6-THA cells demonstrated a faster rate of calcium removal. C6 cells express mRNA from all five subtypes of Da receptors as demonstrated by real time PCR. D1 receptors were most abundant in C6 cells and its expression was further increased in C6-THA cells. Blocking D1-like receptors in C6-THA cells with the specific antagonist drug SCH-23390 induced a decrease in intracellular calcium removal rate, resembling non-manipulated C6 cells' calcium clearance. Da release by C6-THA cells could be related to calcium dependent mechanisms. Furthermore, production of Da by C6-THA cells seems to upregulate the expression of D1 receptors' mRNA.",
keywords = "Dopamine receptors, Fluo-3, Glial cells, L-745,870, Neurotransmitter release, SCH-23390",
author = "{Guerrero Cazares}, Hugo and Alatorre-Carranza, {Mar{\'i}a del Pilar} and Vidal Delgado-Rizo and Duenas-Jimenez, {Judith Marcela} and Mendoza-Magana, {Maria Luisa} and Alberto Morales-Villagran and Ramirez-Herrera, {Mario A.} and Agustin Guerrero-Hern{\'a}ndez and Jose Segovia and Duenas-Jimenez, {Sergio H.}",
year = "2007",
month = "9",
day = "14",
doi = "10.1016/j.brainresbull.2007.05.008",
language = "English (US)",
volume = "74",
pages = "113--118",
journal = "Brain Research Bulletin",
issn = "0361-9230",
publisher = "Elsevier Inc.",
number = "1-3",

}

TY - JOUR

T1 - Dopamine release modifies intracellular calcium levels in tyrosine hydroxylase-transfected C6 cells

AU - Guerrero Cazares, Hugo

AU - Alatorre-Carranza, María del Pilar

AU - Delgado-Rizo, Vidal

AU - Duenas-Jimenez, Judith Marcela

AU - Mendoza-Magana, Maria Luisa

AU - Morales-Villagran, Alberto

AU - Ramirez-Herrera, Mario A.

AU - Guerrero-Hernández, Agustin

AU - Segovia, Jose

AU - Duenas-Jimenez, Sergio H.

PY - 2007/9/14

Y1 - 2007/9/14

N2 - Glioma cell line C6, transfected with tyrosine hydroxylase (TH) cDNA under the control of the glial fibrillary acid protein promoter (C6-THA cells), elicited a reduction in the apomorphine-induced turning behavior when they are implanted in Parkinson's disease models. Nevertheless, dopamine (Da) release has not been explicitly demonstrated nor has a possible mechanism of release been implicated. In this study, the in vitro Da release by C6 and C6-THA cells after chemical stimulation with KCl or glutamate was quantified using HPLC. Modifications in intracellular calcium levels in response to KCl stimulation and participation of Da receptor-mediated feedback in calcium regulation were also studied using FLUO 3 as a calcium concentration indicator. C6-THA cells release dopamine in basal conditions, and increase its release after KCl or glutamic acid stimulation. In a fraction of C6 and C6-THA cells, a transient intracellular calcium increase was observed after KCl stimulation, but C6-THA cells demonstrated a faster rate of calcium removal. C6 cells express mRNA from all five subtypes of Da receptors as demonstrated by real time PCR. D1 receptors were most abundant in C6 cells and its expression was further increased in C6-THA cells. Blocking D1-like receptors in C6-THA cells with the specific antagonist drug SCH-23390 induced a decrease in intracellular calcium removal rate, resembling non-manipulated C6 cells' calcium clearance. Da release by C6-THA cells could be related to calcium dependent mechanisms. Furthermore, production of Da by C6-THA cells seems to upregulate the expression of D1 receptors' mRNA.

AB - Glioma cell line C6, transfected with tyrosine hydroxylase (TH) cDNA under the control of the glial fibrillary acid protein promoter (C6-THA cells), elicited a reduction in the apomorphine-induced turning behavior when they are implanted in Parkinson's disease models. Nevertheless, dopamine (Da) release has not been explicitly demonstrated nor has a possible mechanism of release been implicated. In this study, the in vitro Da release by C6 and C6-THA cells after chemical stimulation with KCl or glutamate was quantified using HPLC. Modifications in intracellular calcium levels in response to KCl stimulation and participation of Da receptor-mediated feedback in calcium regulation were also studied using FLUO 3 as a calcium concentration indicator. C6-THA cells release dopamine in basal conditions, and increase its release after KCl or glutamic acid stimulation. In a fraction of C6 and C6-THA cells, a transient intracellular calcium increase was observed after KCl stimulation, but C6-THA cells demonstrated a faster rate of calcium removal. C6 cells express mRNA from all five subtypes of Da receptors as demonstrated by real time PCR. D1 receptors were most abundant in C6 cells and its expression was further increased in C6-THA cells. Blocking D1-like receptors in C6-THA cells with the specific antagonist drug SCH-23390 induced a decrease in intracellular calcium removal rate, resembling non-manipulated C6 cells' calcium clearance. Da release by C6-THA cells could be related to calcium dependent mechanisms. Furthermore, production of Da by C6-THA cells seems to upregulate the expression of D1 receptors' mRNA.

KW - Dopamine receptors

KW - Fluo-3

KW - Glial cells

KW - L-745,870

KW - Neurotransmitter release

KW - SCH-23390

UR - http://www.scopus.com/inward/record.url?scp=34547549162&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34547549162&partnerID=8YFLogxK

U2 - 10.1016/j.brainresbull.2007.05.008

DO - 10.1016/j.brainresbull.2007.05.008

M3 - Article

C2 - 17683796

AN - SCOPUS:34547549162

VL - 74

SP - 113

EP - 118

JO - Brain Research Bulletin

JF - Brain Research Bulletin

SN - 0361-9230

IS - 1-3

ER -