Dopamine-induced conformational changes in alpha-synuclein

Tiago F. Outerio, Jochen Klucken, Kathryn Bercury, Julie Tetzlaff, Preeti Putcha, Luis M.A. Oliveira, Alexandre Quintas, Pamela J. McLean, Bradley T. Hyman

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

Background: Oligomerization and aggregation of α-synuclein molecules play a major role in neuronal dysfunction and loss in Parkinson's disease. However, α-synuclein oligomerization and aggregation have mostly been detected indirectly in cells using detergent extraction methods. A number of in vitro studies showed that dopamine can modulate the aggregation of α-synuclein by inhibiting the formation of or by disaggregating amyloid fibrils. Methodology/Principal Findings: Here, we show that α-synuclein adopts a variety of conformations in primary neuronal cultures using fluorescence lifetime imaging microscopy (FLIM). Importantly, we found that dopamine, but not dopamine agonists, induced conformational changes in a-synuclein which could be prevented by blocking dopamine transport into the cell. Dopamine also induced conformational changes in α-synuclein expressed in neuronal cell lines, and these changes were also associated with alterations in oligomeric/aggregated species. Conclusion/Significance: Our results show, for the first time, a direct effect of dopamine on the conformation of α-synuclein in neurons, which may help explain the increased vulnerability of dopaminergic neurons in Parkinson's disease.

Original languageEnglish (US)
Article numbere6906
JournalPloS one
Volume4
Issue number9
DOIs
StatePublished - Sep 4 2009

ASJC Scopus subject areas

  • General

Fingerprint

Dive into the research topics of 'Dopamine-induced conformational changes in alpha-synuclein'. Together they form a unique fingerprint.

Cite this