Donor cell type can influence the epigenome and differentiation potential of human induced pluripotent stem cells

Kitai Kim, Rui Zhao, Akiko Doi, Kitwa Ng, Juli Unternaehrer, Patrick Cahan, Huo Hongguang, Yuin Han Loh, Martin J. Aryee, M. William Lensch, Hu Li, James J. Collins, Andrew P. Feinberg, George Q. Daley

Research output: Contribution to journalArticle

365 Scopus citations

Abstract

We compared bona fide human induced pluripotent stem cells (iPSCs) derived from umbilical cord blood (CB) cells and neonatal keratinocytes (K). As a consequence of both incomplete erasure of tissue-specific methylation and aberrant de novo methylation, CB-iPSCs and K-iPSCs were distinct in genome-wide DNA methylation profiles and differentiation potential. Extended passage of some iPSC clones in culture did not improve their epigenetic resemblance to embryonic stem cells, implying that some human iPSCs retain a residual 'epigenetic memory' of their tissue of origin.

Original languageEnglish (US)
Pages (from-to)1117-1119
Number of pages3
JournalNature biotechnology
Volume29
Issue number12
DOIs
StatePublished - Dec 2011

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

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    Kim, K., Zhao, R., Doi, A., Ng, K., Unternaehrer, J., Cahan, P., Hongguang, H., Loh, Y. H., Aryee, M. J., Lensch, M. W., Li, H., Collins, J. J., Feinberg, A. P., & Daley, G. Q. (2011). Donor cell type can influence the epigenome and differentiation potential of human induced pluripotent stem cells. Nature biotechnology, 29(12), 1117-1119. https://doi.org/10.1038/nbt.2052