Docozsahexaenoic acids activate large conductance calcium- Activated potassium channels via phospholipase C-inositol triphosphate- calcium pathway in normal rat coronary smooth muscle cells

Manqing Sun, Lingling Qian, Shipeng Dang, Ying Wu, Xu Tang, Yuan Ji, Xiangyun Wang, Dayun Xia, Wen Wang, Qiang Chai, Tong D Lu, Ruxing Wang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objective To investigate the mechanisms of docosahexaenoic acids ( DHA ) on activating large conductance calcium- Activated potassium channels ( BK channels) in normal rat coronary smooth muscle cells. Methods Normal coronary smooth muscle cells were isolated by enzyme digestion from Sprague-Dawley rats. BK currents were recorded by patch clamp in whole cell and single channel configurations, respectively. The effects of DHA on cytosolic calcium concentrations were examined by recording the changes of fluorescence intensity ratios. Results DHA ( 1 μmol/1,) could activate BK channels. Open probabilities (NP0) of BK channels at test potential 60 mV, and calcium concentrations in external solution at 0, 0. 01, 0. 1, 1, 3, 10, 50 and 100 μmol/L were 0. 002 7 ± 0. 000 4, 0. 006 0 ± 0.001 4, 0.097 2 ±0.010 6, 0. 137 9 ±0.032 9, 0.468 7 ±0. 163 7, 2.097 1 ±0. 310 4 and 3. 120 4 ± 0. 242 7, respectively ( P < 0. 05 , n = 4 ) . Before DHA perfusion, the fluorescence intensity ratio was 0.51 ±0.01, and the ratios were 0.53 ±0.02 and 0.55 ±0.01 after 0.001 and 0.01 (xmol/L DHA perfusion, respectively ( P > 0. 05 , n >5). The ratios were 0. 64 ± 0. 01 , 0. 65 ± 0. 01 , 0. 70 ± 0. 01, 0. 69 ±0.01, 0.68 ±0.01 and 0.67 ±0.02 after 0.1, 0.3, 1, 3, 5 and 10 μmol/L DHA perfusion, respectively, and EC50 was (0. 04 ± 0. 02) μmol/L( P < 0. 05 , n>4). They were all higher than that before DHA perfusion. After incubating with phospholipase C (PLC) blocker U73122 and inositol triphosphate ( IP3) blocker 2-APB, the ratios were 0. 52 ± 0. 01 and 0. 49 ± 0. 02 on the setting of 0. 1 p.mol/L DHA, respectively. Compared with control group (0. 64 ±0. 01) , the ratios decreased after incubating with blockers (P<0.05, 4). Conclusions Docosahexaenoic acids can activate large conductance calcium- Activated potassium channels by the pathway of PLC-IP3-Ca2+ to increase cytosolic calcium concentration in normal coronary smooth muscle cells, dilate the coronary vessels and bestow protective effects on cardiovascular system.

Original languageEnglish (US)
Pages (from-to)530-535
Number of pages6
JournalChinese Journal of Cardiology
Volume44
Issue number6
DOIs
StatePublished - Jun 24 2016
Externally publishedYes

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Large-Conductance Calcium-Activated Potassium Channels
Docosahexaenoic Acids
Type C Phospholipases
Inositol
Smooth Muscle Myocytes
Calcium
Acids
Perfusion
Cardiovascular System
triphosphoric acid
Sprague Dawley Rats
Digestion
Coronary Vessels
Fluorescence
Control Groups
Enzymes

Keywords

  • Coronary vessels
  • Docosahexaenoic acids
  • Large-conductance calcium-Activated potassium channels
  • Myocytes
  • Smooth muscle

ASJC Scopus subject areas

  • Medicine(all)
  • Cardiology and Cardiovascular Medicine

Cite this

Docozsahexaenoic acids activate large conductance calcium- Activated potassium channels via phospholipase C-inositol triphosphate- calcium pathway in normal rat coronary smooth muscle cells. / Sun, Manqing; Qian, Lingling; Dang, Shipeng; Wu, Ying; Tang, Xu; Ji, Yuan; Wang, Xiangyun; Xia, Dayun; Wang, Wen; Chai, Qiang; Lu, Tong D; Wang, Ruxing.

In: Chinese Journal of Cardiology, Vol. 44, No. 6, 24.06.2016, p. 530-535.

Research output: Contribution to journalArticle

Sun, Manqing ; Qian, Lingling ; Dang, Shipeng ; Wu, Ying ; Tang, Xu ; Ji, Yuan ; Wang, Xiangyun ; Xia, Dayun ; Wang, Wen ; Chai, Qiang ; Lu, Tong D ; Wang, Ruxing. / Docozsahexaenoic acids activate large conductance calcium- Activated potassium channels via phospholipase C-inositol triphosphate- calcium pathway in normal rat coronary smooth muscle cells. In: Chinese Journal of Cardiology. 2016 ; Vol. 44, No. 6. pp. 530-535.
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title = "Docozsahexaenoic acids activate large conductance calcium- Activated potassium channels via phospholipase C-inositol triphosphate- calcium pathway in normal rat coronary smooth muscle cells",
abstract = "Objective To investigate the mechanisms of docosahexaenoic acids ( DHA ) on activating large conductance calcium- Activated potassium channels ( BK channels) in normal rat coronary smooth muscle cells. Methods Normal coronary smooth muscle cells were isolated by enzyme digestion from Sprague-Dawley rats. BK currents were recorded by patch clamp in whole cell and single channel configurations, respectively. The effects of DHA on cytosolic calcium concentrations were examined by recording the changes of fluorescence intensity ratios. Results DHA ( 1 μmol/1,) could activate BK channels. Open probabilities (NP0) of BK channels at test potential 60 mV, and calcium concentrations in external solution at 0, 0. 01, 0. 1, 1, 3, 10, 50 and 100 μmol/L were 0. 002 7 ± 0. 000 4, 0. 006 0 ± 0.001 4, 0.097 2 ±0.010 6, 0. 137 9 ±0.032 9, 0.468 7 ±0. 163 7, 2.097 1 ±0. 310 4 and 3. 120 4 ± 0. 242 7, respectively ( P < 0. 05 , n = 4 ) . Before DHA perfusion, the fluorescence intensity ratio was 0.51 ±0.01, and the ratios were 0.53 ±0.02 and 0.55 ±0.01 after 0.001 and 0.01 (xmol/L DHA perfusion, respectively ( P > 0. 05 , n >5). The ratios were 0. 64 ± 0. 01 , 0. 65 ± 0. 01 , 0. 70 ± 0. 01, 0. 69 ±0.01, 0.68 ±0.01 and 0.67 ±0.02 after 0.1, 0.3, 1, 3, 5 and 10 μmol/L DHA perfusion, respectively, and EC50 was (0. 04 ± 0. 02) μmol/L( P < 0. 05 , n>4). They were all higher than that before DHA perfusion. After incubating with phospholipase C (PLC) blocker U73122 and inositol triphosphate ( IP3) blocker 2-APB, the ratios were 0. 52 ± 0. 01 and 0. 49 ± 0. 02 on the setting of 0. 1 p.mol/L DHA, respectively. Compared with control group (0. 64 ±0. 01) , the ratios decreased after incubating with blockers (P<0.05, 4). Conclusions Docosahexaenoic acids can activate large conductance calcium- Activated potassium channels by the pathway of PLC-IP3-Ca2+ to increase cytosolic calcium concentration in normal coronary smooth muscle cells, dilate the coronary vessels and bestow protective effects on cardiovascular system.",
keywords = "Coronary vessels, Docosahexaenoic acids, Large-conductance calcium-Activated potassium channels, Myocytes, Smooth muscle",
author = "Manqing Sun and Lingling Qian and Shipeng Dang and Ying Wu and Xu Tang and Yuan Ji and Xiangyun Wang and Dayun Xia and Wen Wang and Qiang Chai and Lu, {Tong D} and Ruxing Wang",
year = "2016",
month = "6",
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doi = "10.3760/cma.j.issn.0253-3758.2016.06.014",
language = "English (US)",
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pages = "530--535",
journal = "Chinese Journal of Cardiology",
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TY - JOUR

T1 - Docozsahexaenoic acids activate large conductance calcium- Activated potassium channels via phospholipase C-inositol triphosphate- calcium pathway in normal rat coronary smooth muscle cells

AU - Sun, Manqing

AU - Qian, Lingling

AU - Dang, Shipeng

AU - Wu, Ying

AU - Tang, Xu

AU - Ji, Yuan

AU - Wang, Xiangyun

AU - Xia, Dayun

AU - Wang, Wen

AU - Chai, Qiang

AU - Lu, Tong D

AU - Wang, Ruxing

PY - 2016/6/24

Y1 - 2016/6/24

N2 - Objective To investigate the mechanisms of docosahexaenoic acids ( DHA ) on activating large conductance calcium- Activated potassium channels ( BK channels) in normal rat coronary smooth muscle cells. Methods Normal coronary smooth muscle cells were isolated by enzyme digestion from Sprague-Dawley rats. BK currents were recorded by patch clamp in whole cell and single channel configurations, respectively. The effects of DHA on cytosolic calcium concentrations were examined by recording the changes of fluorescence intensity ratios. Results DHA ( 1 μmol/1,) could activate BK channels. Open probabilities (NP0) of BK channels at test potential 60 mV, and calcium concentrations in external solution at 0, 0. 01, 0. 1, 1, 3, 10, 50 and 100 μmol/L were 0. 002 7 ± 0. 000 4, 0. 006 0 ± 0.001 4, 0.097 2 ±0.010 6, 0. 137 9 ±0.032 9, 0.468 7 ±0. 163 7, 2.097 1 ±0. 310 4 and 3. 120 4 ± 0. 242 7, respectively ( P < 0. 05 , n = 4 ) . Before DHA perfusion, the fluorescence intensity ratio was 0.51 ±0.01, and the ratios were 0.53 ±0.02 and 0.55 ±0.01 after 0.001 and 0.01 (xmol/L DHA perfusion, respectively ( P > 0. 05 , n >5). The ratios were 0. 64 ± 0. 01 , 0. 65 ± 0. 01 , 0. 70 ± 0. 01, 0. 69 ±0.01, 0.68 ±0.01 and 0.67 ±0.02 after 0.1, 0.3, 1, 3, 5 and 10 μmol/L DHA perfusion, respectively, and EC50 was (0. 04 ± 0. 02) μmol/L( P < 0. 05 , n>4). They were all higher than that before DHA perfusion. After incubating with phospholipase C (PLC) blocker U73122 and inositol triphosphate ( IP3) blocker 2-APB, the ratios were 0. 52 ± 0. 01 and 0. 49 ± 0. 02 on the setting of 0. 1 p.mol/L DHA, respectively. Compared with control group (0. 64 ±0. 01) , the ratios decreased after incubating with blockers (P<0.05, 4). Conclusions Docosahexaenoic acids can activate large conductance calcium- Activated potassium channels by the pathway of PLC-IP3-Ca2+ to increase cytosolic calcium concentration in normal coronary smooth muscle cells, dilate the coronary vessels and bestow protective effects on cardiovascular system.

AB - Objective To investigate the mechanisms of docosahexaenoic acids ( DHA ) on activating large conductance calcium- Activated potassium channels ( BK channels) in normal rat coronary smooth muscle cells. Methods Normal coronary smooth muscle cells were isolated by enzyme digestion from Sprague-Dawley rats. BK currents were recorded by patch clamp in whole cell and single channel configurations, respectively. The effects of DHA on cytosolic calcium concentrations were examined by recording the changes of fluorescence intensity ratios. Results DHA ( 1 μmol/1,) could activate BK channels. Open probabilities (NP0) of BK channels at test potential 60 mV, and calcium concentrations in external solution at 0, 0. 01, 0. 1, 1, 3, 10, 50 and 100 μmol/L were 0. 002 7 ± 0. 000 4, 0. 006 0 ± 0.001 4, 0.097 2 ±0.010 6, 0. 137 9 ±0.032 9, 0.468 7 ±0. 163 7, 2.097 1 ±0. 310 4 and 3. 120 4 ± 0. 242 7, respectively ( P < 0. 05 , n = 4 ) . Before DHA perfusion, the fluorescence intensity ratio was 0.51 ±0.01, and the ratios were 0.53 ±0.02 and 0.55 ±0.01 after 0.001 and 0.01 (xmol/L DHA perfusion, respectively ( P > 0. 05 , n >5). The ratios were 0. 64 ± 0. 01 , 0. 65 ± 0. 01 , 0. 70 ± 0. 01, 0. 69 ±0.01, 0.68 ±0.01 and 0.67 ±0.02 after 0.1, 0.3, 1, 3, 5 and 10 μmol/L DHA perfusion, respectively, and EC50 was (0. 04 ± 0. 02) μmol/L( P < 0. 05 , n>4). They were all higher than that before DHA perfusion. After incubating with phospholipase C (PLC) blocker U73122 and inositol triphosphate ( IP3) blocker 2-APB, the ratios were 0. 52 ± 0. 01 and 0. 49 ± 0. 02 on the setting of 0. 1 p.mol/L DHA, respectively. Compared with control group (0. 64 ±0. 01) , the ratios decreased after incubating with blockers (P<0.05, 4). Conclusions Docosahexaenoic acids can activate large conductance calcium- Activated potassium channels by the pathway of PLC-IP3-Ca2+ to increase cytosolic calcium concentration in normal coronary smooth muscle cells, dilate the coronary vessels and bestow protective effects on cardiovascular system.

KW - Coronary vessels

KW - Docosahexaenoic acids

KW - Large-conductance calcium-Activated potassium channels

KW - Myocytes

KW - Smooth muscle

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