DNMT1 and DNMT3B modulate distinct polycomb-mediated histone modifications in colon cancer

Bilian Jin, Bing Yao, Jian Liang Li, C. Robert Fields, Amber L. Delmas, Chen Liu, Keith D Robertson

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

DNA methylation patterns are established and maintained by three DNA methyltransferases (DNMT): DNMT1, DNMT3A, and DNMT3B. Although essential for development, methylation patterns are frequently disrupted in cancer and contribute directly to carcinogenesis. Recent studies linking polycomb group repression complexes (PRC1 and PRC2) to the DNMTs have begun to shed light on how methylation is targeted. We identified previously a panel of genes regulated by DNMT3B. Here, we compare these with known polycomb group targets to show that ∼47% of DNMT3B regulated genes are also bound by PRC1 or PRC2. We chose 44 genes coregulated by DNMT3B and PRC1/PRC2 to test whether these criteria would accurately identify novel targets of epigenetic silencing in colon cancer. Using reverse transcription-PCR, bisulfite genomic sequencing, and pyrosequencing, we show that the majority of these genes are frequently silenced in colorectal cancer cell lines and primary tumors. Some of these, including HAND1, HMX2, and SIX3, repressed cell growth. Finally, we analyzed the histone code, DNMT1, DNMT3B, and PRC2 binding by chromatin immunoprecipitation at epigenetically silenced genes to reveal a novel link between DNMT3B and the mark mediated by PRC1. Taken together, these studies suggest that patterns of epigenetic modifiers and the histone code influence the propensity of a gene to become hypermethylated in cancer and that DNMT3B plays an important role in regulating PRC1 function.

Original languageEnglish (US)
Pages (from-to)7412-7421
Number of pages10
JournalCancer Research
Volume69
Issue number18
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

Histone Code
Colonic Neoplasms
Genes
Epigenomics
Methylation
Chromatin Immunoprecipitation
Methyltransferases
DNA Methylation
Tumor Cell Line
Reverse Transcription
Colorectal Neoplasms
Neoplasms
Carcinogenesis
Polymerase Chain Reaction
DNA
Growth

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

DNMT1 and DNMT3B modulate distinct polycomb-mediated histone modifications in colon cancer. / Jin, Bilian; Yao, Bing; Li, Jian Liang; Fields, C. Robert; Delmas, Amber L.; Liu, Chen; Robertson, Keith D.

In: Cancer Research, Vol. 69, No. 18, 2009, p. 7412-7421.

Research output: Contribution to journalArticle

Jin, Bilian ; Yao, Bing ; Li, Jian Liang ; Fields, C. Robert ; Delmas, Amber L. ; Liu, Chen ; Robertson, Keith D. / DNMT1 and DNMT3B modulate distinct polycomb-mediated histone modifications in colon cancer. In: Cancer Research. 2009 ; Vol. 69, No. 18. pp. 7412-7421.
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