DNA sequence resembling vanA and vanB in the vancomycin-resistant biopesticide Bacillus popilliae

Karen Rippere, Robin Patel, James R. Uhl, Kerryl E. Piper, James M. Steckelberg, Bruce C. Kline, Franklin R. Cockerill, Allan A. Yousten

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The origin of high-level vancomycin resistance in enterococci is unknown. Biopesticidal powders containing spores of Bacillus popilliae, which is vancomycin-resistant, have been used for >50 years in the United States for suppression of Japanese beetle populations. Using a polymerase chain reaction assay designed to amplify the vanB gene in enterococci, an amplicon in B. popilliae was identified and sequenced. The putative ligase gene in B. popilliae had 76.8% and 68.4%-68.9% nucleotide identity to the sequences of the vanA and vanB genes, respectively. There was 75.3% and 69.3%-69.9% identity between the translation of the putative ligase gene in B. popilliae and the translation of the vanA and vanB genes, respectively. We have identified a gene resembling vanA and vanB in B. popilliae. The gene in B. popilliae may have been a precursor to or have had an ancestral gene in common with vancomycin resistance genes in enterococci.

Original languageEnglish (US)
Pages (from-to)584-588
Number of pages5
JournalJournal of Infectious Diseases
Volume178
Issue number2
StatePublished - 1998

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Biological Control Agents
Vancomycin
Bacillus
Genes
Enterococcus
Vancomycin Resistance
Ligases
Beetles
Spores
Powders
Nucleotides

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Rippere, K., Patel, R., Uhl, J. R., Piper, K. E., Steckelberg, J. M., Kline, B. C., ... Yousten, A. A. (1998). DNA sequence resembling vanA and vanB in the vancomycin-resistant biopesticide Bacillus popilliae. Journal of Infectious Diseases, 178(2), 584-588.

DNA sequence resembling vanA and vanB in the vancomycin-resistant biopesticide Bacillus popilliae. / Rippere, Karen; Patel, Robin; Uhl, James R.; Piper, Kerryl E.; Steckelberg, James M.; Kline, Bruce C.; Cockerill, Franklin R.; Yousten, Allan A.

In: Journal of Infectious Diseases, Vol. 178, No. 2, 1998, p. 584-588.

Research output: Contribution to journalArticle

Rippere, K, Patel, R, Uhl, JR, Piper, KE, Steckelberg, JM, Kline, BC, Cockerill, FR & Yousten, AA 1998, 'DNA sequence resembling vanA and vanB in the vancomycin-resistant biopesticide Bacillus popilliae', Journal of Infectious Diseases, vol. 178, no. 2, pp. 584-588.
Rippere, Karen ; Patel, Robin ; Uhl, James R. ; Piper, Kerryl E. ; Steckelberg, James M. ; Kline, Bruce C. ; Cockerill, Franklin R. ; Yousten, Allan A. / DNA sequence resembling vanA and vanB in the vancomycin-resistant biopesticide Bacillus popilliae. In: Journal of Infectious Diseases. 1998 ; Vol. 178, No. 2. pp. 584-588.
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