DNA sequence resembling vanA and vanB in the vancomycin-resistant biopesticide Bacillus popilliae

Karen Rippere, Robin Patel, James R. Uhl, Kerryl E. Piper, James M. Steckelberg, Bruce C. Kline, Franklin R. Cockerill, Allan A. Yousten

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19 Scopus citations

Abstract

The origin of high-level vancomycin resistance in enterococci is unknown. Biopesticidal powders containing spores of Bacillus popilliae, which is vancomycin-resistant, have been used for >50 years in the United States for suppression of Japanese beetle populations. Using a polymerase chain reaction assay designed to amplify the vanB gene in enterococci, an amplicon in B. popilliae was identified and sequenced. The putative ligase gene in B. popilliae had 76.8% and 68.4%-68.9% nucleotide identity to the sequences of the vanA and vanB genes, respectively. There was 75.3% and 69.3%-69.9% identity between the translation of the putative ligase gene in B. popilliae and the translation of the vanA and vanB genes, respectively. We have identified a gene resembling vanA and vanB in B. popilliae. The gene in B. popilliae may have been a precursor to or have had an ancestral gene in common with vancomycin resistance genes in enterococci.

Original languageEnglish (US)
Pages (from-to)584-588
Number of pages5
JournalJournal of Infectious Diseases
Volume178
Issue number2
DOIs
StatePublished - Jan 1 1998

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Rippere, K., Patel, R., Uhl, J. R., Piper, K. E., Steckelberg, J. M., Kline, B. C., Cockerill, F. R., & Yousten, A. A. (1998). DNA sequence resembling vanA and vanB in the vancomycin-resistant biopesticide Bacillus popilliae. Journal of Infectious Diseases, 178(2), 584-588. https://doi.org/10.1086/517480