DNA sequence analysis of I-A(β) mutants reveals serologically immunodominant region

B. N. Beck, Larry R Pease, M. P. Bell, J. M. Buerstedde, A. E. Nilson, G. G. Schlauder, D. J. McKean

Research output: Contribution to journalArticle

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Abstract

We have produced a series of in vitro serologically selected cell lines that express mutant I-A molecules. In this report we describe the DNA sequence analysis of the A(β) gene of four cell lines that express serologically altered A(β) polypeptides in association with wild-type A(α) polypeptides. Each of the major serologic epitopes on the A(β) polypeptide has been altered in one or more of the four mutants. In addition, the four mutants exhibit a broad spectrum of functional defects when used to stimulate a panel of T hybridomas of various specificities. The DNA sequence analysis revealed that each mutant had sustained a single nucleotide substitution resulting in a single amino acid substitution. All four independent substitutions occurred within or near the third of the four variable regions defined in the β1 domain of the A(β) polypeptide by allelic comparisons. These data strongly suggest that the third variable region is the major determinant of alloantigenicity on the A(β) polypeptide.

Original languageEnglish (US)
Pages (from-to)433-443
Number of pages11
JournalJournal of Experimental Medicine
Volume166
Issue number2
StatePublished - 1987

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Immunodominant Epitopes
DNA Sequence Analysis
Peptides
Cell Line
Hybridomas
Amino Acid Substitution
Epitopes
Nucleotides
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Beck, B. N., Pease, L. R., Bell, M. P., Buerstedde, J. M., Nilson, A. E., Schlauder, G. G., & McKean, D. J. (1987). DNA sequence analysis of I-A(β) mutants reveals serologically immunodominant region. Journal of Experimental Medicine, 166(2), 433-443.

DNA sequence analysis of I-A(β) mutants reveals serologically immunodominant region. / Beck, B. N.; Pease, Larry R; Bell, M. P.; Buerstedde, J. M.; Nilson, A. E.; Schlauder, G. G.; McKean, D. J.

In: Journal of Experimental Medicine, Vol. 166, No. 2, 1987, p. 433-443.

Research output: Contribution to journalArticle

Beck, BN, Pease, LR, Bell, MP, Buerstedde, JM, Nilson, AE, Schlauder, GG & McKean, DJ 1987, 'DNA sequence analysis of I-A(β) mutants reveals serologically immunodominant region', Journal of Experimental Medicine, vol. 166, no. 2, pp. 433-443.
Beck BN, Pease LR, Bell MP, Buerstedde JM, Nilson AE, Schlauder GG et al. DNA sequence analysis of I-A(β) mutants reveals serologically immunodominant region. Journal of Experimental Medicine. 1987;166(2):433-443.
Beck, B. N. ; Pease, Larry R ; Bell, M. P. ; Buerstedde, J. M. ; Nilson, A. E. ; Schlauder, G. G. ; McKean, D. J. / DNA sequence analysis of I-A(β) mutants reveals serologically immunodominant region. In: Journal of Experimental Medicine. 1987 ; Vol. 166, No. 2. pp. 433-443.
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