DNA Sequence Amplification in Human Prostate Cancer Identified by Chromosome Microdissection: Potential Prognostic Implications

Carla Van Den Berg, Daniel Von Hoff, Michael Sarosdy, Xin Yuan Guan, Michael Bittner, Paul Meltzer, Jeffrey Trent, Robert Brian Jenkins, John Herath, Constance Griffin, Jonathan Epstein, Olio Kallioniemi, Tapio Visakorpi, Daniel Von Hoff, John McGill

Research output: Contribution to journalArticle

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Abstract

The primary aim of this report was to examine the significance of increased DNA sequence copy number (gene amplification) in human prostate cancers. Three methodologies (chromosome microdissection, comparative genomic hybridization, and fluorescence in situ hybridization) were combined to (a) identify a common region of gene amplification in human prostate cells and (b) evaluate in patient samples the prevalence of this genetic change in both primary and recurrent prostate samples. The results of chromosome microdissection revealed a common amplified band region (8q24.1-24.2) in two prostate cases with cytological evidence of gene amplification (double minutes). Fluorescence in situ hybridization using the 8q microdissection probe was performed on fresh tumor touch preparations from 44 randomly selected prostatectomy specimens. Amplification of DNA sequences from 8q24 was observed in 4 (9%) of 44 cases. Four of the 44 patients in this series presented with a positive lymph node at initial diagnosis and 3 of these 4 patients showed 8q amplification. Because of this finding, comparative genomic hybridization and fluorescence in situ hybridization were performed on tumor cells from nine prostate cancer patients with recurrent disease. In eight of nine cases a gain of DNA sequences encompassing 8q24 was observed. Taken together with other evidence implicating 8q gain in prostate cancer progression, these results suggest that the analysis of this genetic change may have diagnostic utility as a marker of prostate cancer progression.

Original languageEnglish (US)
Pages (from-to)11-18
Number of pages8
JournalClinical Cancer Research
Volume1
Issue number1
StatePublished - Jan 1 1995

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Microdissection
Gene Amplification
Prostatic Neoplasms
Chromosomes
Fluorescence In Situ Hybridization
Prostate
Comparative Genomic Hybridization
In Situ Hybridization
Touch
Prostatectomy
Neoplasms
Lymph Nodes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Van Den Berg, C., Von Hoff, D., Sarosdy, M., Guan, X. Y., Bittner, M., Meltzer, P., ... McGill, J. (1995). DNA Sequence Amplification in Human Prostate Cancer Identified by Chromosome Microdissection: Potential Prognostic Implications. Clinical Cancer Research, 1(1), 11-18.

DNA Sequence Amplification in Human Prostate Cancer Identified by Chromosome Microdissection : Potential Prognostic Implications. / Van Den Berg, Carla; Von Hoff, Daniel; Sarosdy, Michael; Guan, Xin Yuan; Bittner, Michael; Meltzer, Paul; Trent, Jeffrey; Jenkins, Robert Brian; Herath, John; Griffin, Constance; Epstein, Jonathan; Kallioniemi, Olio; Visakorpi, Tapio; Von Hoff, Daniel; McGill, John.

In: Clinical Cancer Research, Vol. 1, No. 1, 01.01.1995, p. 11-18.

Research output: Contribution to journalArticle

Van Den Berg, C, Von Hoff, D, Sarosdy, M, Guan, XY, Bittner, M, Meltzer, P, Trent, J, Jenkins, RB, Herath, J, Griffin, C, Epstein, J, Kallioniemi, O, Visakorpi, T, Von Hoff, D & McGill, J 1995, 'DNA Sequence Amplification in Human Prostate Cancer Identified by Chromosome Microdissection: Potential Prognostic Implications', Clinical Cancer Research, vol. 1, no. 1, pp. 11-18.
Van Den Berg C, Von Hoff D, Sarosdy M, Guan XY, Bittner M, Meltzer P et al. DNA Sequence Amplification in Human Prostate Cancer Identified by Chromosome Microdissection: Potential Prognostic Implications. Clinical Cancer Research. 1995 Jan 1;1(1):11-18.
Van Den Berg, Carla ; Von Hoff, Daniel ; Sarosdy, Michael ; Guan, Xin Yuan ; Bittner, Michael ; Meltzer, Paul ; Trent, Jeffrey ; Jenkins, Robert Brian ; Herath, John ; Griffin, Constance ; Epstein, Jonathan ; Kallioniemi, Olio ; Visakorpi, Tapio ; Von Hoff, Daniel ; McGill, John. / DNA Sequence Amplification in Human Prostate Cancer Identified by Chromosome Microdissection : Potential Prognostic Implications. In: Clinical Cancer Research. 1995 ; Vol. 1, No. 1. pp. 11-18.
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