DNA repair following incorporation of 5-fluorouracil into DNA of mouse bone marrow cells

J. D. Schuetz, H. J. Wallace, Robert B Diasio

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

5-Fluorouracil (FUra) was previously demonstrated to be incorporated into DNA at cytotoxic concentrations in mouse bone marrow cells. Subsequently, we showed that under these conditions FUra exhibited a time-dependent removal from DNA accompanied by a decrease in DNA strand length. In the present study we utilized hydroxyurea to inhibit semiconservative DNA synthesis while monitoring DNA repair by measuring the incorporation of [ 3H]dThd into double-stranded DNA (DNAds), which can be separated from DNA at the replication fork (DNAss) by benzoylated-napthoylated-DEAE cellulose. Under these conditions we assessed DNA repair in cells that had previously been exposed for 1 h to varying cytotoxic concentrations of FUra. The results demonstrate an increase in labelling of DNAds with increasing FUra concentration, with no appreciable increase in incorporation of label into DNAss. In conclusion, this study demonstrates that DNA repair occurs following incorporation of FUra. The failure to repair DNA damage at higher FUra concentrations may have a role in the cytotoxicity of this drug.

Original languageEnglish (US)
Pages (from-to)208-210
Number of pages3
JournalCancer Chemotherapy and Pharmacology
Volume21
Issue number3
StatePublished - 1988
Externally publishedYes

Fingerprint

Fluorouracil
DNA Repair
Bone Marrow Cells
Bone
Repair
Cells
DNA
Hydroxyurea
DNA Replication
DNA Damage
Cytotoxicity
Labeling
Labels
Pharmaceutical Preparations
Monitoring

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pharmacology

Cite this

DNA repair following incorporation of 5-fluorouracil into DNA of mouse bone marrow cells. / Schuetz, J. D.; Wallace, H. J.; Diasio, Robert B.

In: Cancer Chemotherapy and Pharmacology, Vol. 21, No. 3, 1988, p. 208-210.

Research output: Contribution to journalArticle

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