DNA conjugated SWCNTs enter endothelial cells via Rac1 mediated macropinocytosis

Santanu Bhattacharya, Daniel Roxbury, Xun Gong, Debabrata Mukhopadhyay, Anand Jagota

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

Several applications of single-walled carbon nanotubes (SWCNT) as nanovectors in biological systems have been reported, and several molecular pathways of cellular entry have been proposed. We employed transmission electron microscopy, confocal fluorescent microscopy, and UV-vis spectroscopic analysis to confirm the internalization of DNA-SWCNT in human umbilical vein endothelial cells. Additionally, by using pharmacological inhibitors as well as genetic approaches, we have found that SWCNT is endocytosed through Rac1- GTPase mediated macropinocytosis in normal endothelial cells.

Original languageEnglish (US)
Pages (from-to)1826-1830
Number of pages5
JournalNano Letters
Volume12
Issue number4
DOIs
StatePublished - Apr 11 2012

Keywords

  • DNA-SWCNT
  • HUVEC
  • endothelial
  • internalization
  • nanotubes
  • pinocytosis

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

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