DNA conjugated SWCNTs enter endothelial cells via Rac1 mediated macropinocytosis

Santanu Bhattacharya; Daniel Roxbury; Xun Gong; Debabrata Mukhopadhyay; Anand Jagota

doi:10.1021/nl204058u

DNA conjugated SWCNTs enter endothelial cells via Rac1 mediated macropinocytosis

Santanu Bhattacharya, Daniel Roxbury, Xun Gong, Debabrata Mukhopadhyay, Anand Jagota

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Several applications of single-walled carbon nanotubes (SWCNT) as nanovectors in biological systems have been reported, and several molecular pathways of cellular entry have been proposed. We employed transmission electron microscopy, confocal fluorescent microscopy, and UV-vis spectroscopic analysis to confirm the internalization of DNA-SWCNT in human umbilical vein endothelial cells. Additionally, by using pharmacological inhibitors as well as genetic approaches, we have found that SWCNT is endocytosed through Rac1- GTPase mediated macropinocytosis in normal endothelial cells.

Original language	English (US)
Pages (from-to)	1826-1830
Number of pages	5
Journal	Nano Letters
Volume	12
Issue number	4
DOIs	https://doi.org/10.1021/nl204058u
State	Published - Apr 11 2012

Keywords

DNA-SWCNT
HUVEC
endothelial
internalization
nanotubes
pinocytosis

ASJC Scopus subject areas

Bioengineering
Chemistry(all)
Materials Science(all)
Condensed Matter Physics
Mechanical Engineering

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10.1021/nl204058u

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title = "DNA conjugated SWCNTs enter endothelial cells via Rac1 mediated macropinocytosis",

abstract = "Several applications of single-walled carbon nanotubes (SWCNT) as nanovectors in biological systems have been reported, and several molecular pathways of cellular entry have been proposed. We employed transmission electron microscopy, confocal fluorescent microscopy, and UV-vis spectroscopic analysis to confirm the internalization of DNA-SWCNT in human umbilical vein endothelial cells. Additionally, by using pharmacological inhibitors as well as genetic approaches, we have found that SWCNT is endocytosed through Rac1- GTPase mediated macropinocytosis in normal endothelial cells.",

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author = "Santanu Bhattacharya and Daniel Roxbury and Xun Gong and Debabrata Mukhopadhyay and Anand Jagota",

year = "2012",

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T1 - DNA conjugated SWCNTs enter endothelial cells via Rac1 mediated macropinocytosis

AU - Bhattacharya, Santanu

AU - Roxbury, Daniel

AU - Gong, Xun

AU - Mukhopadhyay, Debabrata

AU - Jagota, Anand

PY - 2012/4/11

Y1 - 2012/4/11

N2 - Several applications of single-walled carbon nanotubes (SWCNT) as nanovectors in biological systems have been reported, and several molecular pathways of cellular entry have been proposed. We employed transmission electron microscopy, confocal fluorescent microscopy, and UV-vis spectroscopic analysis to confirm the internalization of DNA-SWCNT in human umbilical vein endothelial cells. Additionally, by using pharmacological inhibitors as well as genetic approaches, we have found that SWCNT is endocytosed through Rac1- GTPase mediated macropinocytosis in normal endothelial cells.

AB - Several applications of single-walled carbon nanotubes (SWCNT) as nanovectors in biological systems have been reported, and several molecular pathways of cellular entry have been proposed. We employed transmission electron microscopy, confocal fluorescent microscopy, and UV-vis spectroscopic analysis to confirm the internalization of DNA-SWCNT in human umbilical vein endothelial cells. Additionally, by using pharmacological inhibitors as well as genetic approaches, we have found that SWCNT is endocytosed through Rac1- GTPase mediated macropinocytosis in normal endothelial cells.

KW - DNA-SWCNT

KW - HUVEC

KW - endothelial

KW - internalization

KW - nanotubes

KW - pinocytosis

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JO - Nano Letters

JF - Nano Letters

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ER -

DNA conjugated SWCNTs enter endothelial cells via Rac1 mediated macropinocytosis

Abstract

Keywords

ASJC Scopus subject areas

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