DNA bridging and looping by HMO1 provides a mechanism for stabilizing nucleosome-free chromatin

Divakaran Murugesapillai, Micah J. McCauley, Ran Huo, Molly H.Nelson Holte, Armen Stepanyants, L. James Maher, Nathan E. Israeloff, Mark C. Williams

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

The regulation of chromatin structure in eukaryotic cells involves abundant architectural factors such as high mobility group B (HMGB) proteins. It is not understood how these factors control the interplay between genome accessibility and compaction. In vivo, HMO1 binds the promoter and coding regions of most ribosomal RNA genes, facilitating transcription and possibly stabilizing chromatin in the absence of histones. To understand how HMO1 performs these functions, we combine single molecule stretching and atomic force microscopy (AFM). By stretching HMO1-bound DNA, we demonstrate a hierarchical organization of interactions, in which HMO1 initially compacts DNA on a timescale of seconds, followed by bridge formation and stabilization of DNA loops on a timescale of minutes. AFM experiments demonstrate DNA bridging between strands as well as looping by HMO1. Our results support a model in which HMO1 maintains the stability of nucleosome-free chromatin regions by forming complex and dynamic DNA structures mediated by protein-protein interactions.

Original languageEnglish (US)
Pages (from-to)8996-9004
Number of pages9
JournalNucleic acids research
Volume42
Issue number14
DOIs
StatePublished - 2014

ASJC Scopus subject areas

  • Genetics

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    Murugesapillai, D., McCauley, M. J., Huo, R., Holte, M. H. N., Stepanyants, A., Maher, L. J., Israeloff, N. E., & Williams, M. C. (2014). DNA bridging and looping by HMO1 provides a mechanism for stabilizing nucleosome-free chromatin. Nucleic acids research, 42(14), 8996-9004. https://doi.org/10.1093/nar/gku635