DNA Binding of TEA/ATTS Domain Factors Is Regulated by Protein Kinase C Phosphorylation in Human Choriocarcinoma Cells

Shi Wen Jiang, Maoqing Dong, Miguel A. Trujillo, Laurence J Miller, Norman L. Eberhardt

Research output: Contribution to journalArticle

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Abstract

Transcription enhancer factor 1 (TEF-1) controls the expression of a diverse set of genes. Previous studies implicated protein kinase C (PKC)-mediated signal transduction in modulating TEF function. We demonstrate that in human choriocarcinoma BeWo cells, the PKC activator 12-O-tetradecanoyl phorbol 13-acetate and PKC inhibitor bisindolylmaleimide reciprocally down- and up-regulate, respectively, TEF-mediated GGAATG core enhancer activity. In vitro TEF-1 phosphorylation with several PKC isozymes and phosphoamino acid analysis confirmed that TEF-1 is a potential PKC substrate. TEF-1·DNA complexes formed by BeWo nuclear extracts are supershifted by phosphoserine- and phosphothreonine- but not phosphotyrosine-specific antibodies, indicating that TEF-1 is phosphorylated in vivo at serine and threonine residues. The TEF-1 phosphorylation domain was localized to the third α-helix of the DNA binding domain and adjacent hinge region by phosphopeptide analysis. TEF-1 phosphorylation significantly reduced its DNA binding activity both in vitro and in vivo, providing a possible mechanism for the inhibitory action of PKC. Finally, BeWo cells contained abundant levels of γ and δ PKC isoforms, and their overexpression resulted in even greater inhibition of GGAATG core enhancer activity after 12-O-tetradecanoyl phorbol 13-acetate treatment. These data strongly suggest that PKC-mediated phosphorylation is a key factor controlling TEF function.

Original languageEnglish (US)
Pages (from-to)23464-23470
Number of pages7
JournalJournal of Biological Chemistry
Volume276
Issue number26
DOIs
StatePublished - Jun 29 2001

Fingerprint

Choriocarcinoma
Phosphorylation
Protein Kinase C
Transcription factors
Transcription Factors
DNA
Tetradecanoylphorbol Acetate
Acetate Kinase
Phosphothreonine
Phosphoamino Acids
Phosphoserine
Phosphopeptides
Signal transduction
Phosphotyrosine
Protein C Inhibitor
Threonine
Hinges
Protein Kinase Inhibitors
Serine
Isoenzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

DNA Binding of TEA/ATTS Domain Factors Is Regulated by Protein Kinase C Phosphorylation in Human Choriocarcinoma Cells. / Jiang, Shi Wen; Dong, Maoqing; Trujillo, Miguel A.; Miller, Laurence J; Eberhardt, Norman L.

In: Journal of Biological Chemistry, Vol. 276, No. 26, 29.06.2001, p. 23464-23470.

Research output: Contribution to journalArticle

Jiang, Shi Wen ; Dong, Maoqing ; Trujillo, Miguel A. ; Miller, Laurence J ; Eberhardt, Norman L. / DNA Binding of TEA/ATTS Domain Factors Is Regulated by Protein Kinase C Phosphorylation in Human Choriocarcinoma Cells. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 26. pp. 23464-23470.
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