DNA bending by charged peptides: Electrophoretic and spectroscopic analyses

Robert J. McDonald, Anatoly I. Dragan, William R. Kirk, Kevin L. Neff, Peter L. Privalov, L. James Maher

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

We are testing the idea that placement of fixed charges near one face of the DNA double helix can induce DNA bending by a purely electrostatic mechanism. If stretching forces between DNA phosphates are significant, fixed charges should induce DNA bending by asymmetrically modulating these forces. We have previously tested this hypothesis by adding charged residues to small bZIP DNA binding peptides and monitoring DNA bending using electrophoretic phasing assays. Our results were consistent with an electrostatic model of DNA bending in predicted directions. We now confirm these observations with fluorescence resonance energy transfer (FRET). Using a "U"-shaped DNA probe, we report that DNA bending by charged bZIP peptides is readily detected by FRET. We further show that charged bZIP peptides cause DNA bending rather than DNA twisting.

Original languageEnglish (US)
Pages (from-to)2306-2316
Number of pages11
JournalBiochemistry
Volume46
Issue number9
DOIs
StatePublished - Mar 6 2007

ASJC Scopus subject areas

  • Biochemistry

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