DNA bending by bHLH charge variants

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We wish to understand the role of electrostatics in DNA stiffness and bending. The DNA charge collapse model suggests that mutual electrostatic repulsions between neighboring phosphates significantly contribute to DNA stiffness. According to this model, placement of fixed charges near the negatively charged DNA surface should induce bending through asymmetric reduction or enhancement of these inter-phosphate repulsive forces. We have reported previously that charged variants of the elongated basic-leucine zipper (bZIP) domain of Gcn4p bend DNA in a manner consistent with this charge collapse model. To extend this result to a more globular protein, we present an investigation of the dimeric basic-helix-loop-helix (bHLH) domain of Pho4p. The 62 amino acid bHLH domain has been modified to position charged amino acid residues near one face of the DNA double helix. As observed for bZIP charge variants, DNA bending toward appended cations (away from the protein:DNA interface) is observed. However, unlike bZIP proteins, DNA is not bent away from bHLH anionic charges. This finding can be explained by the structure of the more globular bHLH domain which, in contrast to bZIP proteins, makes extensive DNA contacts along the binding face.

Original languageEnglish (US)
Pages (from-to)4846-4856
Number of pages11
JournalNucleic Acids Research
Volume34
Issue number17
DOIs
StatePublished - Oct 2006

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DNA
Leucine Zippers
Helix-Loop-Helix Motifs
Static Electricity
Proteins
Phosphates
Basic Amino Acids
Cations
Amino Acids

ASJC Scopus subject areas

  • Genetics

Cite this

DNA bending by bHLH charge variants. / McDonald, Robert; Kahn, Jason D.; Maher III, L James.

In: Nucleic Acids Research, Vol. 34, No. 17, 10.2006, p. 4846-4856.

Research output: Contribution to journalArticle

McDonald, Robert ; Kahn, Jason D. ; Maher III, L James. / DNA bending by bHLH charge variants. In: Nucleic Acids Research. 2006 ; Vol. 34, No. 17. pp. 4846-4856.
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