Distribution of Y-Receptors in Murine Lingual Epithelia

Maria D. Hurtado, Andres Acosta, Paola P. Riveros, Bruce J. Baum, Kirill Ukhanov, Alicia R. Brown, Cedrick D. Dotson, Herbert Herzog, Sergei Zolotukhin

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Peptide hormones and their cognate receptors belonging to neuropeptide Y (NPY) family mediate diverse biological functions in a number of tissues. Recently, we discovered the presence of the gut satiation peptide YY (PYY) in saliva of mice and humans and defined its role in the regulation of food intake and body weight maintenance. Here we report the systematic analysis of expression patterns of all NPY receptors (Rs), Y1R, Y2R, Y4R, and Y5R in lingual epithelia in mice. Using four independent assays, immunohistochemistry, in situ hybridization, immunocytochemistry and RT PCR, we show that the morphologically different layers of the keratinized stratified epithelium of the dorsal layer of the tongue express Y receptors in a very distinctive yet overlapping pattern. In particular, the monolayer of basal progenitor cells expresses both Y1 and Y2 receptors. Y1Rs are present in the parabasal prickle cell layer and the granular layer, while differentiated keratinocytes display abundant Y5Rs. Y4Rs are expressed substantially in the neuronal fibers innervating the lamina propria and mechanoreceptors. Basal epithelial cells positive for Y2Rs respond robustly to PYY3-36 by increasing intracellular Ca2+ suggesting their possible functional interaction with salivary PYY. In taste buds of the circumvallate papillae, some taste receptor cells (TRCs) express YRs localized primarily at the apical domain, indicative of their potential role in taste perception. Some of the YR-positive TRCs are co-localized with neuronal cell adhesion molecule (NCAM), suggesting that these TRCs may have synaptic contacts with nerve terminals. In summary, we show that all YRs are abundantly expressed in multiple lingual cell types, including epithelial progenitors, keratinocytes, neuronal dendrites and TRCs. These results suggest that these receptors may be involved in the mediation of a wide variety of functions, including proliferation, differentiation, motility, taste perception and satiation.

Original languageEnglish (US)
Article numbere46358
JournalPloS one
Volume7
Issue number9
DOIs
StatePublished - Sep 26 2012
Externally publishedYes

Fingerprint

Peptide YY
tongue
Tongue
Neuronal Cell Adhesion Molecules
epithelium
Epithelium
Neuropeptide Y Receptors
Salivary Proteins and Peptides
receptors
Taste Perception
Peptide Hormones
Neuropeptide Y
mice
Satiation
peptide YY
Monolayers
Assays
neuropeptide Y
Keratinocytes
keratinocytes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Hurtado, M. D., Acosta, A., Riveros, P. P., Baum, B. J., Ukhanov, K., Brown, A. R., ... Zolotukhin, S. (2012). Distribution of Y-Receptors in Murine Lingual Epithelia. PloS one, 7(9), [e46358]. https://doi.org/10.1371/journal.pone.0046358

Distribution of Y-Receptors in Murine Lingual Epithelia. / Hurtado, Maria D.; Acosta, Andres; Riveros, Paola P.; Baum, Bruce J.; Ukhanov, Kirill; Brown, Alicia R.; Dotson, Cedrick D.; Herzog, Herbert; Zolotukhin, Sergei.

In: PloS one, Vol. 7, No. 9, e46358, 26.09.2012.

Research output: Contribution to journalArticle

Hurtado, MD, Acosta, A, Riveros, PP, Baum, BJ, Ukhanov, K, Brown, AR, Dotson, CD, Herzog, H & Zolotukhin, S 2012, 'Distribution of Y-Receptors in Murine Lingual Epithelia', PloS one, vol. 7, no. 9, e46358. https://doi.org/10.1371/journal.pone.0046358
Hurtado MD, Acosta A, Riveros PP, Baum BJ, Ukhanov K, Brown AR et al. Distribution of Y-Receptors in Murine Lingual Epithelia. PloS one. 2012 Sep 26;7(9). e46358. https://doi.org/10.1371/journal.pone.0046358
Hurtado, Maria D. ; Acosta, Andres ; Riveros, Paola P. ; Baum, Bruce J. ; Ukhanov, Kirill ; Brown, Alicia R. ; Dotson, Cedrick D. ; Herzog, Herbert ; Zolotukhin, Sergei. / Distribution of Y-Receptors in Murine Lingual Epithelia. In: PloS one. 2012 ; Vol. 7, No. 9.
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