Distribution of noncollagenous proteins in the matrix of adult human bone: Evidence of anatomic and functional heterogeneity

Ronald T. Ingram, Bart Clarke, Larry W. Fisher, Lorraine A. Fitzpatrick

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Abstract

The microanatomic distribution of several noncollagenous proteins (NCPs) in bone matrix was examined by immunohistochemical analysis of glycol‐methyl methacrylate‐embedded normal adult human bone biopsies. Osteopontin and bone sialoprotein stained throughout the lamellae of both trabecular and cortical bone. Cement lines (cortical and trabecular) and the mineralized matrix immediately adjacent to each Haversian canal were intensely stained. Osteocalcin was detected in cement lines; however, lamellar staining varied depending on the location within the individual unit of bone. In cortical bone, the inner concentric lamellae of osteons were often unstained but the outer lamellae were heavily stained for osteocalcin. Osteonectin was not detected in cement lines and in most specimens revealed a pattern similar to that of osteocalcin with respect to the absence of immunostaining within the inner concentric lamellae. Decorin was prominent in the perilacunar matrix, the canaliculi of osteocytes, and the matrix immediately adjacent to quiescent Haversian canals. Biglycan appeared evenly distributed throughout cortical and trabecular bone matrix. These results suggest that the incorporation of NCPs into matrix may vary depending on the stage of formation of individual bone units. The specific distribution and spatial relationship of these NCPs may be related to the function of each protein during bone resorption and formation. The distinct patterns of NCP localization in bone support the hypothesis that in addition to their structural and mineral‐inducing properties, these proteins may influence the events associated with bone remodeling, such as recruitment, attachment, differentiation, and activity of bone cells.

Original languageEnglish (US)
Pages (from-to)1019-1029
Number of pages11
JournalJournal of Bone and Mineral Research
Volume8
Issue number9
DOIs
StatePublished - Jan 1 1993

Fingerprint

Haversian System
Bone and Bones
Osteocalcin
Bone Matrix
Proteins
Osteogenesis
Biglycan
Osteonectin
Integrin-Binding Sialoprotein
Decorin
Osteocytes
Osteopontin
Bone Remodeling
Bone Resorption
Staining and Labeling
Biopsy
Cortical Bone
Cancellous Bone

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine

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Distribution of noncollagenous proteins in the matrix of adult human bone : Evidence of anatomic and functional heterogeneity. / Ingram, Ronald T.; Clarke, Bart; Fisher, Larry W.; Fitzpatrick, Lorraine A.

In: Journal of Bone and Mineral Research, Vol. 8, No. 9, 01.01.1993, p. 1019-1029.

Research output: Contribution to journalArticle

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