TY - JOUR
T1 - Distribution of endothelin-1 in transplanted human lungs
AU - Jeppsson, Anders
AU - Tazelaar, Henry D.
AU - Miller, Virginia M.
AU - Mcgregor, Christopher G.A.
PY - 1998/9/27
Y1 - 1998/9/27
N2 - Background. This study was designed to assess which cells in transplanted lungs express endothelin-1 (ET-1) and if expression of the peptide can be used to discriminate between rejection and infection in transplanted lungs. Methods. Transbronchial biopsies (n-104) from 29 burnan lung transplant recipients were stained immunohistochemically for ET-1. Cells expressing ET-1 (pneumocytes, endothelial cells, airway epithelial cells, lymphocytes, and macrophages) were quantified and correlated with clinical histopathology findings. Results. ET-1 was expressed in airway epithelial cells (93% of the biopsies), infiltrating macrophages (86%), and lymphocytes (19%) but not in endothelial cells or pneumocytes. ET-1 expression did not vary with rejection, obliterative bronchiolitis, or infection. ET-1 expression did not correlate with age, grade of rejection, pulmonary function, or time after transplantation. Conclusion. In transplanted human lungs, ET-1 is expressed in airway epithelial cells and infiltrating macrophages, and expression does not vary with pathological processes. Therefore, immnunostaining for ET-1 probably cannot be used to discriminate between rejection and infection in transplanted lungs.
AB - Background. This study was designed to assess which cells in transplanted lungs express endothelin-1 (ET-1) and if expression of the peptide can be used to discriminate between rejection and infection in transplanted lungs. Methods. Transbronchial biopsies (n-104) from 29 burnan lung transplant recipients were stained immunohistochemically for ET-1. Cells expressing ET-1 (pneumocytes, endothelial cells, airway epithelial cells, lymphocytes, and macrophages) were quantified and correlated with clinical histopathology findings. Results. ET-1 was expressed in airway epithelial cells (93% of the biopsies), infiltrating macrophages (86%), and lymphocytes (19%) but not in endothelial cells or pneumocytes. ET-1 expression did not vary with rejection, obliterative bronchiolitis, or infection. ET-1 expression did not correlate with age, grade of rejection, pulmonary function, or time after transplantation. Conclusion. In transplanted human lungs, ET-1 is expressed in airway epithelial cells and infiltrating macrophages, and expression does not vary with pathological processes. Therefore, immnunostaining for ET-1 probably cannot be used to discriminate between rejection and infection in transplanted lungs.
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U2 - 10.1097/00007890-199809270-00018
DO - 10.1097/00007890-199809270-00018
M3 - Article
C2 - 9771847
AN - SCOPUS:0032572978
SN - 0041-1337
VL - 66
SP - 806
EP - 809
JO - Transplantation
JF - Transplantation
IS - 6
ER -