Distribution of Cryptosporidium parvum sporozoite apical organelles during attachment to and internalization by cultured biliary epithelial cells

Steven P. O'Hara, Bing Q. Huang, Xian Ming Chen, Jeremy Nelson, Nicholas F. LaRusso

Research output: Contribution to journalArticle

8 Scopus citations


Although accumulating evidence supports an active role for host cells during Cryptosporidium parvum invasion of epithelia, our knowledge of the underlying parasite-specific processes triggering such events is limited. In an effort to better understand the invasion strategy of C. parvum, we characterized the presence and distribution of the apical organelles (micronemes, dense granules, and rhoptry) through the stages of attachment to, and internalization by, human biliary epithelia, using serial-section electron microscopy. Novel findings include an apparent organized rearrangement of micronemes upon host cell attachment. The apically segregated micronemes were apposed to a central microtubule-like filamentous structure, and the more distal micronemes localized to the periphery and apical region of the parasite during internalization, coinciding with the formation of the anterior vacuole. The morphological observations presented here extend our understanding of parasite-specific processes that occur during attachment to, and internalization by, host epithelial cells.

Original languageEnglish (US)
Pages (from-to)995-999
Number of pages5
JournalJournal of Parasitology
Issue number5
StatePublished - Oct 1 2005


ASJC Scopus subject areas

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics

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