Distribution of Cryptosporidium parvum sporozoite apical organelles during attachment to and internalization by cultured biliary epithelial cells

Steven P. O'Hara, Bing Q. Huang, Xian Ming Chen, Jeremy Nelson, Nicholas F La Russo

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Although accumulating evidence supports an active role for host cells during Cryptosporidium parvum invasion of epithelia, our knowledge of the underlying parasite-specific processes triggering such events is limited. In an effort to better understand the invasion strategy of C. parvum, we characterized the presence and distribution of the apical organelles (micronemes, dense granules, and rhoptry) through the stages of attachment to, and internalization by, human biliary epithelia, using serial-section electron microscopy. Novel findings include an apparent organized rearrangement of micronemes upon host cell attachment. The apically segregated micronemes were apposed to a central microtubule-like filamentous structure, and the more distal micronemes localized to the periphery and apical region of the parasite during internalization, coinciding with the formation of the anterior vacuole. The morphological observations presented here extend our understanding of parasite-specific processes that occur during attachment to, and internalization by, host epithelial cells.

Original languageEnglish (US)
Pages (from-to)995-999
Number of pages5
JournalJournal of Parasitology
Volume91
Issue number5
DOIs
StatePublished - Oct 2005

Fingerprint

Cryptosporidium parvum
Sporozoites
sporozoites
Organelles
organelles
parasite
Parasites
epithelial cells
Epithelial Cells
parasites
epithelium
Epithelium
Vacuoles
electron microscopy
Microtubules
microtubules
vacuoles
granules
Electron Microscopy
cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Parasitology
  • Microbiology

Cite this

Distribution of Cryptosporidium parvum sporozoite apical organelles during attachment to and internalization by cultured biliary epithelial cells. / O'Hara, Steven P.; Huang, Bing Q.; Chen, Xian Ming; Nelson, Jeremy; La Russo, Nicholas F.

In: Journal of Parasitology, Vol. 91, No. 5, 10.2005, p. 995-999.

Research output: Contribution to journalArticle

@article{8d8b23246515421785b328092b110afe,
title = "Distribution of Cryptosporidium parvum sporozoite apical organelles during attachment to and internalization by cultured biliary epithelial cells",
abstract = "Although accumulating evidence supports an active role for host cells during Cryptosporidium parvum invasion of epithelia, our knowledge of the underlying parasite-specific processes triggering such events is limited. In an effort to better understand the invasion strategy of C. parvum, we characterized the presence and distribution of the apical organelles (micronemes, dense granules, and rhoptry) through the stages of attachment to, and internalization by, human biliary epithelia, using serial-section electron microscopy. Novel findings include an apparent organized rearrangement of micronemes upon host cell attachment. The apically segregated micronemes were apposed to a central microtubule-like filamentous structure, and the more distal micronemes localized to the periphery and apical region of the parasite during internalization, coinciding with the formation of the anterior vacuole. The morphological observations presented here extend our understanding of parasite-specific processes that occur during attachment to, and internalization by, host epithelial cells.",
author = "O'Hara, {Steven P.} and Huang, {Bing Q.} and Chen, {Xian Ming} and Jeremy Nelson and {La Russo}, {Nicholas F}",
year = "2005",
month = "10",
doi = "10.1645/GE-495R.1",
language = "English (US)",
volume = "91",
pages = "995--999",
journal = "Journal of Parasitology",
issn = "0022-3395",
publisher = "American Society of Parasitologists",
number = "5",

}

TY - JOUR

T1 - Distribution of Cryptosporidium parvum sporozoite apical organelles during attachment to and internalization by cultured biliary epithelial cells

AU - O'Hara, Steven P.

AU - Huang, Bing Q.

AU - Chen, Xian Ming

AU - Nelson, Jeremy

AU - La Russo, Nicholas F

PY - 2005/10

Y1 - 2005/10

N2 - Although accumulating evidence supports an active role for host cells during Cryptosporidium parvum invasion of epithelia, our knowledge of the underlying parasite-specific processes triggering such events is limited. In an effort to better understand the invasion strategy of C. parvum, we characterized the presence and distribution of the apical organelles (micronemes, dense granules, and rhoptry) through the stages of attachment to, and internalization by, human biliary epithelia, using serial-section electron microscopy. Novel findings include an apparent organized rearrangement of micronemes upon host cell attachment. The apically segregated micronemes were apposed to a central microtubule-like filamentous structure, and the more distal micronemes localized to the periphery and apical region of the parasite during internalization, coinciding with the formation of the anterior vacuole. The morphological observations presented here extend our understanding of parasite-specific processes that occur during attachment to, and internalization by, host epithelial cells.

AB - Although accumulating evidence supports an active role for host cells during Cryptosporidium parvum invasion of epithelia, our knowledge of the underlying parasite-specific processes triggering such events is limited. In an effort to better understand the invasion strategy of C. parvum, we characterized the presence and distribution of the apical organelles (micronemes, dense granules, and rhoptry) through the stages of attachment to, and internalization by, human biliary epithelia, using serial-section electron microscopy. Novel findings include an apparent organized rearrangement of micronemes upon host cell attachment. The apically segregated micronemes were apposed to a central microtubule-like filamentous structure, and the more distal micronemes localized to the periphery and apical region of the parasite during internalization, coinciding with the formation of the anterior vacuole. The morphological observations presented here extend our understanding of parasite-specific processes that occur during attachment to, and internalization by, host epithelial cells.

UR - http://www.scopus.com/inward/record.url?scp=28444439865&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=28444439865&partnerID=8YFLogxK

U2 - 10.1645/GE-495R.1

DO - 10.1645/GE-495R.1

M3 - Article

C2 - 16419739

AN - SCOPUS:28444439865

VL - 91

SP - 995

EP - 999

JO - Journal of Parasitology

JF - Journal of Parasitology

SN - 0022-3395

IS - 5

ER -