TY - JOUR
T1 - Distribution of 1,25-dihydroxyvitamin D3 receptor immunoreactivity in the rat brain and spinal cord
AU - Prüfer, Kirsten
AU - Veenstra, Timothy D.
AU - Jirikowski, Gustav F.
AU - Kumar, Rajiv
N1 - Funding Information:
Supported by NIGH grant DK 25409 to RK, by DFG grant Ji 10/4-2 and by the Thüringer Ministerium für Wissenschaft, Forschung und Kultur (Project B301-96095).
PY - 1999/2
Y1 - 1999/2
N2 - A complete mapping study on the 1,25-dihydroxyvitamin D3 receptor immunoreactivity within the rat central nervous system was performed with a monoclonal and a polyclonal antibody. Specific immunostaining was observed within both nuclear and cytoplasmic compartments of a variety of cells in the cerebellum, mesopontine area, diencephalon, cortex, spinal cord, and limbic system. Both monoclonal and polyclonal antibodies provided similar staining patterns. The monoclonal antibody stained distinct domains within the nuclei of all and the cytoplasm of specific neuronal cell types, like motor neurons, Purkinje cells, and pyramidal cells of the cortex more clearly than the polyclonal antibody. The expression of vitamin D3 receptor in the rat central nervous system was confirmed by in situ hybridisation. The widespread distribution of vitamin D3 receptor in distinct portions of the sensory, motor, and limbic brain systems suggests multiple functional properties of 1,25-dihydroxyvitamin D3 in the central nervous system.
AB - A complete mapping study on the 1,25-dihydroxyvitamin D3 receptor immunoreactivity within the rat central nervous system was performed with a monoclonal and a polyclonal antibody. Specific immunostaining was observed within both nuclear and cytoplasmic compartments of a variety of cells in the cerebellum, mesopontine area, diencephalon, cortex, spinal cord, and limbic system. Both monoclonal and polyclonal antibodies provided similar staining patterns. The monoclonal antibody stained distinct domains within the nuclei of all and the cytoplasm of specific neuronal cell types, like motor neurons, Purkinje cells, and pyramidal cells of the cortex more clearly than the polyclonal antibody. The expression of vitamin D3 receptor in the rat central nervous system was confirmed by in situ hybridisation. The widespread distribution of vitamin D3 receptor in distinct portions of the sensory, motor, and limbic brain systems suggests multiple functional properties of 1,25-dihydroxyvitamin D3 in the central nervous system.
KW - Brain
KW - Calcitriol
KW - Immunohistochemistry
KW - Steroids
KW - VDR
UR - http://www.scopus.com/inward/record.url?scp=2542508588&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2542508588&partnerID=8YFLogxK
U2 - 10.1016/S0891-0618(99)00002-2
DO - 10.1016/S0891-0618(99)00002-2
M3 - Article
C2 - 10223312
AN - SCOPUS:2542508588
SN - 0891-0618
VL - 16
SP - 135
EP - 145
JO - Journal of Chemical Neuroanatomy
JF - Journal of Chemical Neuroanatomy
IS - 2
ER -