Distribution of 1,25-dihydroxyvitamin D3 receptor immunoreactivity in the rat brain and spinal cord

Kirsten Prüfer, Timothy D. Veenstra, Gustav F. Jirikowski, Rajiv Kumar

Research output: Contribution to journalArticlepeer-review

194 Scopus citations

Abstract

A complete mapping study on the 1,25-dihydroxyvitamin D3 receptor immunoreactivity within the rat central nervous system was performed with a monoclonal and a polyclonal antibody. Specific immunostaining was observed within both nuclear and cytoplasmic compartments of a variety of cells in the cerebellum, mesopontine area, diencephalon, cortex, spinal cord, and limbic system. Both monoclonal and polyclonal antibodies provided similar staining patterns. The monoclonal antibody stained distinct domains within the nuclei of all and the cytoplasm of specific neuronal cell types, like motor neurons, Purkinje cells, and pyramidal cells of the cortex more clearly than the polyclonal antibody. The expression of vitamin D3 receptor in the rat central nervous system was confirmed by in situ hybridisation. The widespread distribution of vitamin D3 receptor in distinct portions of the sensory, motor, and limbic brain systems suggests multiple functional properties of 1,25-dihydroxyvitamin D3 in the central nervous system.

Original languageEnglish (US)
Pages (from-to)135-145
Number of pages11
JournalJournal of Chemical Neuroanatomy
Volume16
Issue number2
DOIs
StatePublished - Feb 1999

Keywords

  • Brain
  • Calcitriol
  • Immunohistochemistry
  • Steroids
  • VDR

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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