Distribution and quantitation of immunoreactive gut neuropeptides in piebald mice and their normal littermates

Bruce H. Kaufman, Timothy R. Koch, Joseph H. Szurszewski, Vay Liang W Go

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Piebald mice inherit congenital megacolon associated with distal aganglionosis. To study the distribution of intrinsic peptidergic nerves in the gut of piebald mice and their normal littermates, we used specific radioimmunoassays to measure the tissue concentrations of the following immunoreactive neuropeptides: vasoactive intestinal peptide (VIP), peptide histidine-isoleucine (PHI), [Met]enkephalin (Enk), substance P (SP), and bombesin-like intestinal peptide (BLIP). In the normal littermates, all neuropeptide concentrations were significantly greater in the colon than in the proximal gut. SP, Enk, VIP, PHI, and BLIP levels were all decreased in the distal colon of piebald mice as compared to normal littermates. SP, BLIP, and Enk levels were also decreased in the dilated proximal colon of piebald mice. These results suggest that there are abnormalities in the peptidergic innervation of the proximal and the distal colon in piebald mice. The abnormalities localized to the proximal colon of piebald mice may be related to functional obstruction of the colon.

Original languageEnglish (US)
Pages (from-to)479-483
Number of pages5
JournalJournal of Surgical Research
Volume38
Issue number5
DOIs
StatePublished - 1985

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Neuropeptides
Colon
Bombesin
Substance P
Peptide PHI
Enkephalins
Vasoactive Intestinal Peptide
Peptides
Hirschsprung Disease
Methionine Enkephalin
Radioimmunoassay

ASJC Scopus subject areas

  • Surgery

Cite this

Distribution and quantitation of immunoreactive gut neuropeptides in piebald mice and their normal littermates. / Kaufman, Bruce H.; Koch, Timothy R.; Szurszewski, Joseph H.; Go, Vay Liang W.

In: Journal of Surgical Research, Vol. 38, No. 5, 1985, p. 479-483.

Research output: Contribution to journalArticle

Kaufman, Bruce H. ; Koch, Timothy R. ; Szurszewski, Joseph H. ; Go, Vay Liang W. / Distribution and quantitation of immunoreactive gut neuropeptides in piebald mice and their normal littermates. In: Journal of Surgical Research. 1985 ; Vol. 38, No. 5. pp. 479-483.
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