Distinguishing lung tumours from normal lung based on a small set of genes

Tatiana Dracheva, Reena Philip, Wenming Xiao, Alexander G. Gee, John McCarthy, Ping Yang, Yue Wang, Gang Dong, Hongjun Yang, Jin Jen

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Identifying specific molecular markers and developing sensitive detection methods are two of the fundamental requirements for detection and differential diagnosis of cancer. Toward this goal, we first performed cDNA array analysis using 65 non-small cell lung cancer and non-involved normal lung tissues. We then used several complementary statistical and analytical methods to examine gene expression profiles generated by us and others from four independent sets of normal and neoplastic lung tissues. We report here that several sets of roughly 20 genes were sufficient to provide a robust distinction between normal and neoplastic tissues of the lung. Next we assessed the predictive ability of these gene sets by using Flow-Thru Chips® (FTC) (MetriGenix, Baltimore, MD) containing 20 genes to screen 48 primary lung tumours and normal lung tissues. Gene expression changes detected by FTC distinguished lung cancers from the normal lung tissues using an RNA amount equivalent to that present in as few as 300 cells. We also used an independent set of 24 genes and showed that their expression profile was equally effective when measured by quantitative polymerase chain reaction (Q-PCR). Our results demonstrate that lung cancers can be identified based on the expression patterns of just 20 genes and that this approach is applicable for cancer diagnosis, prognosis, and monitoring using small amount of tumour or biopsy samples.

Original languageEnglish (US)
Pages (from-to)157-164
Number of pages8
JournalLung Cancer
Volume55
Issue number2
DOIs
StatePublished - Feb 2007

Fingerprint

Lung
Lung Neoplasms
Genes
Neoplasms
Baltimore
Oligonucleotide Array Sequence Analysis
Transcriptome
Non-Small Cell Lung Carcinoma
Differential Diagnosis
RNA
Biopsy
Gene Expression
Polymerase Chain Reaction

Keywords

  • Cancer detection
  • Class prediction
  • Expression profiling
  • Flow-Thru Chip
  • Gene chip
  • Non-small cell lung cancer

ASJC Scopus subject areas

  • Oncology

Cite this

Dracheva, T., Philip, R., Xiao, W., Gee, A. G., McCarthy, J., Yang, P., ... Jen, J. (2007). Distinguishing lung tumours from normal lung based on a small set of genes. Lung Cancer, 55(2), 157-164. https://doi.org/10.1016/j.lungcan.2006.10.025

Distinguishing lung tumours from normal lung based on a small set of genes. / Dracheva, Tatiana; Philip, Reena; Xiao, Wenming; Gee, Alexander G.; McCarthy, John; Yang, Ping; Wang, Yue; Dong, Gang; Yang, Hongjun; Jen, Jin.

In: Lung Cancer, Vol. 55, No. 2, 02.2007, p. 157-164.

Research output: Contribution to journalArticle

Dracheva, T, Philip, R, Xiao, W, Gee, AG, McCarthy, J, Yang, P, Wang, Y, Dong, G, Yang, H & Jen, J 2007, 'Distinguishing lung tumours from normal lung based on a small set of genes', Lung Cancer, vol. 55, no. 2, pp. 157-164. https://doi.org/10.1016/j.lungcan.2006.10.025
Dracheva, Tatiana ; Philip, Reena ; Xiao, Wenming ; Gee, Alexander G. ; McCarthy, John ; Yang, Ping ; Wang, Yue ; Dong, Gang ; Yang, Hongjun ; Jen, Jin. / Distinguishing lung tumours from normal lung based on a small set of genes. In: Lung Cancer. 2007 ; Vol. 55, No. 2. pp. 157-164.
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