TY - JOUR
T1 - Distinguishing between hepatic inflammation and fibrosis with MR elastography
AU - Yin, Meng
AU - Glaser, Kevin J.
AU - Manduca, Armando
AU - Mounajjed, Taofic
AU - Malhi, Harmeet
AU - Simonetto, Douglas A.
AU - Wang, Ruisi
AU - Yang, Liu
AU - Mao, Shennen A.
AU - Glorioso, Jaime M.
AU - Elgilani, Faysal M.
AU - Ward, Christopher J.
AU - Harris, Peter C.
AU - Nyberg, Scott L.
AU - Shah, Vijay H.
AU - Ehman, Richard L.
N1 - Funding Information:
ities related to the present article: disclosed no relevant relationships. Activities not related to the present article: receives royalties from and holds stock in Resoundant. Other relationships: has intellectual property rights and a financial interest in MR elastography technology. K.J.G. Activities related to the present article: disclosed no relevant relationships. Activities not related to the present article: holds stock in Re-soundant, has patents and intellectual property related to the technology used in this research, and receive royalties from the licensing of this technology. Other relationships: disclosed no relevant relationships. A.M. Activities related to the present article: disclosed no relevant relationships. Activities not related to the present article: received nonfinancial support from Re-soundant. Other relationships: disclosed no relevant relationships. T.M. disclosed no relevant relationships. H.M. disclosed no relevant relationships. D.A.S. disclosed no relevant relationships. R.W. disclosed no relevant relationships. L.Y. disclosed no relevant relationships. S.A.M. disclosed no relevant relationships. J.M.G. disclosed no relevant relationships. F.M.E. disclosed no relevant relationships. C.J.W. disclosed no relevant relationships. P.C.H. Activ- ities related to the present article: disclosed no relevant relationships. Activities not related to the present article: received a grant from Otsuka Pharmaceuticals. Other relationships: disclosed no relevant relationships. S.L.N. disclosed no relevant relationships. V.H.S. Activities related to the present article: disclosed no relevant relationships. Activities not related to the present article: has intellectual property rights and a financial interest in MR elastography. Other relationships: disclosed no relevant relationships. R.L.E. Activities related to the present article: disclosed no relevant relationships. Activities not related to the present article: received a grant from and holds patents issued by Resoun-dant. Other relationships: serves as CEO of Resoundant.
Funding Information:
ane M. Sauter, RT (R) (MR); Usman Yaqoob, MBBS; and Anuradha Krishnan, PhD, for their excellent technical assistance. One of the mouse models (ARPKD) was provided by Mayo Translational PKD Center (DK090728).
PY - 2017/9
Y1 - 2017/9
N2 - Purpose: To investigate the utility of magnetic resonance (MR) elastography- derived mechanical properties in the discrimination of hepatic inflammation and fibrosis in the early stages of chronic liver diseases. Materials and Methods: All studies were approved by the institutional animal care and use committee. A total of 187 animals were studied, including 182 mice and five pigs. These animals represented five different liver diseases with a varying combination and extent of hepatic inflammation, fibrosis, congestion, and portal hypertension. Multifrequency three-dimensional MR elastography was performed, and shear stiffness, storage modulus, shear loss modulus, and damping ratio were calculated for all animals. Necroinflammation, fibrosis, and portal pressure were either histologically scored or biochemically and physically quantified in all animals. Two-sided Welch t tests were used to evaluate mean differences between disease and control groups. Spearman correlation analyses were used to evaluate the relationships between mechanical parameters and quantitative fibrosis extent (hydroxyproline concentration) and portal pressure. Results: Liver stiffness and storage modulus increased with progressively developed fibrosis and portal hypertension (mean stiffness at 80 Hz and 48-week feeding, 0.51 kPa±0.12 in the steatohepatitis group vs 0.29 kPa±0.01 in the control group; P = .02). Damping ratio and shear loss modulus can be used to distinguish inflammation from fibrosis at early stages of disease, even before the development of histologically detectable necroinflammation and fibrosis (mean damping ratio at 80 Hz and 20- week feeding, 0.044±0.012 in the steatohepatitis group vs 0.014±0.008 in the control group; P lt;.001). Damping ratio and liver stiffness vary differently with respect to cause of portal hypertension (ie, congestion- or cirrhosisinduced hypertension). These differentiation abilities have frequency-dependent variations. Conclusion: Liver stiffness and damping ratio measurements can extend hepatic MR elastography to potentially enable assessment of necroinflammatory, congestive, and fibrotic processes of chronic liver diseases.
AB - Purpose: To investigate the utility of magnetic resonance (MR) elastography- derived mechanical properties in the discrimination of hepatic inflammation and fibrosis in the early stages of chronic liver diseases. Materials and Methods: All studies were approved by the institutional animal care and use committee. A total of 187 animals were studied, including 182 mice and five pigs. These animals represented five different liver diseases with a varying combination and extent of hepatic inflammation, fibrosis, congestion, and portal hypertension. Multifrequency three-dimensional MR elastography was performed, and shear stiffness, storage modulus, shear loss modulus, and damping ratio were calculated for all animals. Necroinflammation, fibrosis, and portal pressure were either histologically scored or biochemically and physically quantified in all animals. Two-sided Welch t tests were used to evaluate mean differences between disease and control groups. Spearman correlation analyses were used to evaluate the relationships between mechanical parameters and quantitative fibrosis extent (hydroxyproline concentration) and portal pressure. Results: Liver stiffness and storage modulus increased with progressively developed fibrosis and portal hypertension (mean stiffness at 80 Hz and 48-week feeding, 0.51 kPa±0.12 in the steatohepatitis group vs 0.29 kPa±0.01 in the control group; P = .02). Damping ratio and shear loss modulus can be used to distinguish inflammation from fibrosis at early stages of disease, even before the development of histologically detectable necroinflammation and fibrosis (mean damping ratio at 80 Hz and 20- week feeding, 0.044±0.012 in the steatohepatitis group vs 0.014±0.008 in the control group; P lt;.001). Damping ratio and liver stiffness vary differently with respect to cause of portal hypertension (ie, congestion- or cirrhosisinduced hypertension). These differentiation abilities have frequency-dependent variations. Conclusion: Liver stiffness and damping ratio measurements can extend hepatic MR elastography to potentially enable assessment of necroinflammatory, congestive, and fibrotic processes of chronic liver diseases.
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U2 - 10.1148/radiol.2017160622
DO - 10.1148/radiol.2017160622
M3 - Article
C2 - 28128707
AN - SCOPUS:85028348853
VL - 284
SP - 694
EP - 705
JO - Radiology
JF - Radiology
SN - 0033-8419
IS - 3
ER -