Distinctive eosinophilic cytoplasmic inclusion bodies in melanocytic nevi: An immunohistochemical and ultrastructural study

Wonwoo Shon, David A. Wada, Lawrence E. Gibson, Thomas J Flotte, Bernd W. Scheithauer

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: We sought to further determine the histochemical, immunohistochemical and ultrastructural properties of eosinophilic cytoplasmic inclusion bodies in melanocytic nevi. Methods: Skin specimens from four patients with a known diagnosis of conventional melanocytic nevus (3) or Spitz nevus (1) and containing intracytoplasmic eosinophilic inclusion bodies were selected. In addition, melanomas (25), Spitz nevi (10) and blue nevi (4) were examined to determine the frequency of the inclusions. Results: Inclusions tended to be located in multinucleated melanocytes with abundant vacuolated cytoplasm. In conventional (hematoxylin and eosin-stained) sections, the degree of density and eosinophilia of intracytoplasmic inclusions varied with size. Periodic acid-Schiff, Fontana and Congo red stains showed no reactivity. All bodies were immunoreactive for ubiquitin but negative for tyrosinase, keratin and vimentin. Ultrastructurally, inclusion bodies were non-membrane bound, ranged from 4 to 7 μm, and were comprised of radiating filamentous structures with or without an electron-dense core. Electron probe x-ray microanalysis revealed no significant peaks. None of additional melanomas, Spitz nevi and blue nevi that were evaluated showed similar inclusions. Conclusions: The inclusion bodies described herein bear no resemblance to other cytoplasmic inclusion bodies previously described in melanocytic lesions. There is no discernible relationship to melanosomes by ultrastructural analysis. We postulate a relationship with dysfunction of ubiquitin-mediated protein degradation occurring in melanocytes.

Original languageEnglish (US)
Pages (from-to)865-870
Number of pages6
JournalJournal of Cutaneous Pathology
Volume38
Issue number11
DOIs
StatePublished - Nov 2011

Fingerprint

Pigmented Nevus
Inclusion Bodies
Epithelioid and Spindle Cell Nevus
Blue Nevus
Melanocytes
Ubiquitin
lissamine rhodamine B
Melanoma
Electrons
Melanosomes
Congo Red
Periodic Acid
Monophenol Monooxygenase
Eosinophilia
Vimentin
Hematoxylin
Eosine Yellowish-(YS)
Keratins
Proteolysis
Cytoplasm

Keywords

  • electron microscopy
  • eosinophilic cytoplasmic inclusion body
  • melanocytic nevi
  • Spitz nevi

ASJC Scopus subject areas

  • Dermatology
  • Pathology and Forensic Medicine
  • Histology

Cite this

Distinctive eosinophilic cytoplasmic inclusion bodies in melanocytic nevi : An immunohistochemical and ultrastructural study. / Shon, Wonwoo; Wada, David A.; Gibson, Lawrence E.; Flotte, Thomas J; Scheithauer, Bernd W.

In: Journal of Cutaneous Pathology, Vol. 38, No. 11, 11.2011, p. 865-870.

Research output: Contribution to journalArticle

Shon, Wonwoo ; Wada, David A. ; Gibson, Lawrence E. ; Flotte, Thomas J ; Scheithauer, Bernd W. / Distinctive eosinophilic cytoplasmic inclusion bodies in melanocytic nevi : An immunohistochemical and ultrastructural study. In: Journal of Cutaneous Pathology. 2011 ; Vol. 38, No. 11. pp. 865-870.
@article{8b70b074a5ca490bb8802cd22d481aa9,
title = "Distinctive eosinophilic cytoplasmic inclusion bodies in melanocytic nevi: An immunohistochemical and ultrastructural study",
abstract = "Background: We sought to further determine the histochemical, immunohistochemical and ultrastructural properties of eosinophilic cytoplasmic inclusion bodies in melanocytic nevi. Methods: Skin specimens from four patients with a known diagnosis of conventional melanocytic nevus (3) or Spitz nevus (1) and containing intracytoplasmic eosinophilic inclusion bodies were selected. In addition, melanomas (25), Spitz nevi (10) and blue nevi (4) were examined to determine the frequency of the inclusions. Results: Inclusions tended to be located in multinucleated melanocytes with abundant vacuolated cytoplasm. In conventional (hematoxylin and eosin-stained) sections, the degree of density and eosinophilia of intracytoplasmic inclusions varied with size. Periodic acid-Schiff, Fontana and Congo red stains showed no reactivity. All bodies were immunoreactive for ubiquitin but negative for tyrosinase, keratin and vimentin. Ultrastructurally, inclusion bodies were non-membrane bound, ranged from 4 to 7 μm, and were comprised of radiating filamentous structures with or without an electron-dense core. Electron probe x-ray microanalysis revealed no significant peaks. None of additional melanomas, Spitz nevi and blue nevi that were evaluated showed similar inclusions. Conclusions: The inclusion bodies described herein bear no resemblance to other cytoplasmic inclusion bodies previously described in melanocytic lesions. There is no discernible relationship to melanosomes by ultrastructural analysis. We postulate a relationship with dysfunction of ubiquitin-mediated protein degradation occurring in melanocytes.",
keywords = "electron microscopy, eosinophilic cytoplasmic inclusion body, melanocytic nevi, Spitz nevi",
author = "Wonwoo Shon and Wada, {David A.} and Gibson, {Lawrence E.} and Flotte, {Thomas J} and Scheithauer, {Bernd W.}",
year = "2011",
month = "11",
doi = "10.1111/j.1600-0560.2011.01764.x",
language = "English (US)",
volume = "38",
pages = "865--870",
journal = "Journal of Cutaneous Pathology",
issn = "0303-6987",
publisher = "Wiley-Blackwell",
number = "11",

}

TY - JOUR

T1 - Distinctive eosinophilic cytoplasmic inclusion bodies in melanocytic nevi

T2 - An immunohistochemical and ultrastructural study

AU - Shon, Wonwoo

AU - Wada, David A.

AU - Gibson, Lawrence E.

AU - Flotte, Thomas J

AU - Scheithauer, Bernd W.

PY - 2011/11

Y1 - 2011/11

N2 - Background: We sought to further determine the histochemical, immunohistochemical and ultrastructural properties of eosinophilic cytoplasmic inclusion bodies in melanocytic nevi. Methods: Skin specimens from four patients with a known diagnosis of conventional melanocytic nevus (3) or Spitz nevus (1) and containing intracytoplasmic eosinophilic inclusion bodies were selected. In addition, melanomas (25), Spitz nevi (10) and blue nevi (4) were examined to determine the frequency of the inclusions. Results: Inclusions tended to be located in multinucleated melanocytes with abundant vacuolated cytoplasm. In conventional (hematoxylin and eosin-stained) sections, the degree of density and eosinophilia of intracytoplasmic inclusions varied with size. Periodic acid-Schiff, Fontana and Congo red stains showed no reactivity. All bodies were immunoreactive for ubiquitin but negative for tyrosinase, keratin and vimentin. Ultrastructurally, inclusion bodies were non-membrane bound, ranged from 4 to 7 μm, and were comprised of radiating filamentous structures with or without an electron-dense core. Electron probe x-ray microanalysis revealed no significant peaks. None of additional melanomas, Spitz nevi and blue nevi that were evaluated showed similar inclusions. Conclusions: The inclusion bodies described herein bear no resemblance to other cytoplasmic inclusion bodies previously described in melanocytic lesions. There is no discernible relationship to melanosomes by ultrastructural analysis. We postulate a relationship with dysfunction of ubiquitin-mediated protein degradation occurring in melanocytes.

AB - Background: We sought to further determine the histochemical, immunohistochemical and ultrastructural properties of eosinophilic cytoplasmic inclusion bodies in melanocytic nevi. Methods: Skin specimens from four patients with a known diagnosis of conventional melanocytic nevus (3) or Spitz nevus (1) and containing intracytoplasmic eosinophilic inclusion bodies were selected. In addition, melanomas (25), Spitz nevi (10) and blue nevi (4) were examined to determine the frequency of the inclusions. Results: Inclusions tended to be located in multinucleated melanocytes with abundant vacuolated cytoplasm. In conventional (hematoxylin and eosin-stained) sections, the degree of density and eosinophilia of intracytoplasmic inclusions varied with size. Periodic acid-Schiff, Fontana and Congo red stains showed no reactivity. All bodies were immunoreactive for ubiquitin but negative for tyrosinase, keratin and vimentin. Ultrastructurally, inclusion bodies were non-membrane bound, ranged from 4 to 7 μm, and were comprised of radiating filamentous structures with or without an electron-dense core. Electron probe x-ray microanalysis revealed no significant peaks. None of additional melanomas, Spitz nevi and blue nevi that were evaluated showed similar inclusions. Conclusions: The inclusion bodies described herein bear no resemblance to other cytoplasmic inclusion bodies previously described in melanocytic lesions. There is no discernible relationship to melanosomes by ultrastructural analysis. We postulate a relationship with dysfunction of ubiquitin-mediated protein degradation occurring in melanocytes.

KW - electron microscopy

KW - eosinophilic cytoplasmic inclusion body

KW - melanocytic nevi

KW - Spitz nevi

UR - http://www.scopus.com/inward/record.url?scp=80053562288&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80053562288&partnerID=8YFLogxK

U2 - 10.1111/j.1600-0560.2011.01764.x

DO - 10.1111/j.1600-0560.2011.01764.x

M3 - Article

C2 - 21819442

AN - SCOPUS:80053562288

VL - 38

SP - 865

EP - 870

JO - Journal of Cutaneous Pathology

JF - Journal of Cutaneous Pathology

SN - 0303-6987

IS - 11

ER -