Distinct requirements for zebrafish angiogenesis revealed by a VEGF-A morphant

Aidas Nasevicius, Jon Larson, Stephen C Ekker

Research output: Contribution to journalArticle

198 Citations (Scopus)

Abstract

Angiogcnesis is a fundamental vertebrate developmental process that requires signalling by the secreted protein vascular cndothclial growth factor-A (VEGF-A). VEGF-A functions in the development of embryonic structures, during tissue remodelling and for the growth of tumour-induced vasculature. The study of the role of VEGF-A during normal development has been significantly complicated by the dominant, haplo-insufficient nature of VEGF-A-targeted mutations in mice. We have used morpholino-based targeted gene knock-down technology to generate a zebrafish VEGF-A morphant loss of function model. Zebrafish VEGF-A morphant embryos develop with an enlarged pericardium and with major blood vessel deficiencies. Morphological assessment at 2 days of development indicates a nearly complete absence of both axial and interscgmcntal vasculature, with no or reduced numbers of circulating red blood cells. Molecular analysis using the endothelial markers fli-1 and flk-1 at 1 day of development demonstrates a fundamental distinction between VEGF-A requirements for axial and intersegmental vascular structure specification. VEGF-A is not required for the initial establishment of axial vasculature patterning, whereas all development of intersegmental vasculature is dependent on VEGF-A signalling. The zebrafish thus serves as a quality model for the study of conserved vertebrate angiogenesis processes during embryonic development.

Original languageEnglish (US)
Pages (from-to)294-301
Number of pages8
JournalYeast
Volume17
Issue number4
StatePublished - 2000
Externally publishedYes

Fingerprint

vascular endothelial growth factor A
Zebrafish
angiogenesis
Danio rerio
Vascular Endothelial Growth Factor A
Blood vessels
Tumors
Blood
Genes
Cells
Tissue
Proteins
Specifications
blood vessels
Blood Vessels
Vertebrates
Embryonic Structures
embryonic structures
vertebrates
Gene Knockdown Techniques

Keywords

  • Angiogenesis
  • Fli-1
  • Flk-1
  • Morphant
  • Morpholino
  • Vasculature
  • VEGF-A
  • Zebrafish

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Microbiology

Cite this

Distinct requirements for zebrafish angiogenesis revealed by a VEGF-A morphant. / Nasevicius, Aidas; Larson, Jon; Ekker, Stephen C.

In: Yeast, Vol. 17, No. 4, 2000, p. 294-301.

Research output: Contribution to journalArticle

Nasevicius, A, Larson, J & Ekker, SC 2000, 'Distinct requirements for zebrafish angiogenesis revealed by a VEGF-A morphant', Yeast, vol. 17, no. 4, pp. 294-301.
Nasevicius, Aidas ; Larson, Jon ; Ekker, Stephen C. / Distinct requirements for zebrafish angiogenesis revealed by a VEGF-A morphant. In: Yeast. 2000 ; Vol. 17, No. 4. pp. 294-301.
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