Distinct pathways regulate transforming growth factor β1‐stimulated proto‐oncogene and extracellular matrix gene expression

Philip H. Howe, Muriel R. Cunningham, Edward B. Leof

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

The effect of pertussis toxin (PT) on transforming growth factor β1 (TGFβl)‐induced proto‐oncogene expression was investigated in AKR‐2B fibroblasts. PT substantially abolished c‐sis and c‐myc mRNA expression following TGFβl stimulation. This inhibitory effect was specific for TGFβ1‐stimulated proto‐oncogene expression and associated with the ADP‐ribosylation of a 41‐kDa substrate. Actinomycin D decay and nuclear run‐on experiments demonstrated that the inhibitory effects of PT are a result of decreased transcriptional activation and not to an increased decay of proto‐oncogene message. PT did not, however, affect TGFβl‐stimulated fibronectin and collagen mRNA accumulation nor did it have any inhibitory effect on TGFβl‐induced morphological transformation. These data indicate that TGFβl‐stimulated gene expression is coupled to multiple pathways distinguished by their sensitivity to PT.

Original languageEnglish (US)
Pages (from-to)39-45
Number of pages7
JournalJournal of Cellular Physiology
Volume142
Issue number1
DOIs
StatePublished - Jan 1990

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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